^(125)IUdR对胶质瘤细胞靶点放疗后的大鼠生存分析  

Survival analysis of C6 glioma DNA-targeting radio-therapy by Auger electron emitted from^(125)Iudr in rats.

作　　者：罗林[1] 游潮[2] 赵建华[1] 司马秀田[1] 杨雷[1] 柏顺明[1] 盖雪松[1] 郑云飞[1] 张永发[1] 太柏[1] 杨滨[1] 

机构地区：[1]云南省第一人民医院神经外科,云南昆明650032 [2]四川大学华西医院神经外科,四川成都610041

出　　处：《云南医药》2004年第5期370-371,共2页Medicine and Pharmacy of Yunnan

基　　金：云南省自然科学基金资助项目 (NO . 1999CO112M)

摘　　要：目的　在建立C6 Wistar胶质瘤大鼠模型的基础上 ,研究1 2 5IUdR介导俄歇电子释放对大鼠C6胶质瘤DNA靶点放疗后的大鼠生存分析。方法　建立C6 Wistar胶质瘤大鼠模型后 ,将Wistar大鼠分为实验组 (2 0只 )、对照组 (2 0只 )和空白组 (10只 ) 3组 ,前两组再分为 5日处死和生存分析两个亚组。实验组在肿瘤增殖高峰期于肿瘤接种原位分次注入1 2 5IUdR ,0 2mCi 10 μl 次 ;对照组同法注入等摩尔浓度的1 2 7IUdR ,5 6 μM 10 μl 次 ;空白组同法洲入等量生理盐水 ,10 μl 次。了解1 2 5IUdR和1 2 7IUdR治疗后 3组动物的生存情况。结果　在 5 0天的观察期内 ,实验组有 2只生存下来 ,实验组中期生存时间比空白组和对照组延长了 1倍多 (P <0 0 5 )。Objective To investigate the action and mechanism of Auger electron emitting from^(125)Iudr on DNA-targeting radiotherapy of C6 glioma in rat based on establishment of a C6/Wistar glioma model.Methods A total of 50 male Wistar rats were randomizely divided into three groups:Experimental group(n=20),control group(n=20)and sham group(n=10).The animals of two former groups were separted into two subgroups:5 day sacrificing group and survival analysis group.Experimental group was infused intracerebrally^(125)Iudr per 8 hour during proliferation peak(0.2mCi/10μl/time),total dosage was 0.6mCi/rat.Control group were infused intracerebrally^(127)Iudr per 8 hour(5.6um/10μl/time),total dosage was 16.8μm/rat.Sham group were infused with 10μl of 0.9% saline.Animal survival was followed overtime.Results 1.Gross pathological examination demonstrated tumor weight and diameter in experimental group were lower than control group(P<0.05).2.Two rats treated with^(125)Iudr(26 days)were markedly longer than that of control animals treated with^(127)Iudr(12 days)(P<0.05).Conclusions ^(125)Iudr is cell cycle dependent drug which can only be taken in by cells of cell division stage.So that this drug is harmless to the healthy cells in CNS and then is helpful and valuable in clinical application.

关 键 词：^125IUDR C6胶质瘤细胞 生存分析 俄歇电子 

分 类 号：R651[医药卫生—外科学]