二十二碳六烯酸乙酯对脑缺血再灌注氧化损伤及脑水肿的影响  

Protective effects of ethyl docosahexaenoate on brain oxidative injury and edema induced by cerebral ischemic reperfusion

作　　者：薛任皓[1] 曹德华[1] 郑维发[2] 孙靓[1] 刘志礼[1] 

机构地区：[1]南京大学生物系,江苏南京210093 [2]徐州师范大学药用植物重点实验室,江苏徐州221116

出　　处：《中国海洋药物》2004年第5期21-25,共5页Chinese Journal of Marine Drugs

摘　　要：目的研究二十二碳六烯酸乙酯 (ethyldocosahexaenoate ,E DHA)对沙土鼠脑缺血再灌注引起的氧化损伤和脑水肿的保护作用。方法采用沙土鼠双侧颈总动脉阻断法制作全脑缺血再灌注损伤模型 ,检测E DHA对缺血再灌注后脑中丙二醛 (MDA)和谷胱甘肽 (GSH)含量 ;谷胱甘肽过氧化物酶 (GSH Px)、过氧化氢酶 (CAT)、超氧化物歧化酶 (SOD)、ATP酶活性 ;水及Na+ 、Ca2 + 含量的影响 ,并进行了病理检查。结果缺血前E DHA预防给药 10周有效阻止了脑缺血再灌注引起的MDA升高 ,GSH降低 ,GSH Px、CAT活性降低 ,ATP酶活性降低 ,水及Ca2 + 、Na+ 含量升高。结论E DHA对沙土鼠脑缺血再灌注具有保护作用 ,其机理与清除自由基和减轻脑水肿有关。Objective To investigate the protective effects of ethyl docosahexaenoate on brain oxidative injury and edema induced by cerebral ischemic/reperfusion in gerbils. Methods The gerbils were subjected to both common carotid arteries occlusion. The contents of MDA and GSH, the activities of GSH-PX, CAT, SOD and ATPase, the water content and the concentrations of Na + and Ca 2+ were measured. Histopathological examination was also done. Results The pretreatment with E-DHA significantly prevented the raise of MDA level, the decline of GSH content, the activities of GSH-Px, CAT, ATPase and the increases of Ca 2+, Na + and water content. Conclusion E-DHA has protective effect on brain ischemic injury and edema, which may be due to an inhibitory action on hydroxyl radical formation and brain edema.

关 键 词：二十二碳六烯酸乙酯 脑缺血再灌注损伤 自由基 脑水肿 

分 类 号：R931.77[医药卫生—生药学] R972[医药卫生—药学]