PPARα激动剂对培养的人单核细胞表达MIP-1α的影响  

Effect of PPARα Agonist on the Expression of Macrophage Inflammatory Protein-1α in Cultured Human Monocytes

作　　者：田丹[1] 朱大和[1] 冯艳[1] 瞿智玲[1] 

机构地区：[1]华中科技大学同济医学院基础医学院病理学系,武汉430030

出　　处：《华中科技大学学报（医学版）》2004年第5期535-537,F004,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的　探讨过氧化物酶体增殖剂激活受体α (PPARα)的激活对单核细胞处于正常状态或脂质过氧化损伤时表达巨噬细胞炎性蛋白 1α (MIP 1α)的影响。方法　将培养的单核细胞分为 :①对照组 ;②联胺组 ;③不同浓度的Wy14 6 4 3组 ;④不同浓度的Wy14 6 4 3+联胺组 ;⑤不同作用时间Wy14 6 4 3组 ;⑥不同作用时间Wy14 6 4 3+联胺组。免疫细胞化学法检测各组细胞MIP 1α蛋白的表达。结果　暴露于联胺后 ,人单核细胞MIP 1α蛋白的表达增强(P <0 0 1) ;PPARα的激动剂在有或无脂质过氧化损伤时均能明显诱导人单核细胞MIP 1α蛋白的表达 (P <0 0 1) ,但随着时间的延长可有下降。结论　PPARα参与了MIP 1α蛋白在单核细胞中表达的调节从而参与了动脉粥样硬化的发生。Objective To investigate the effects of PPARα agonist on the expression of macrophage inflammatory protein-1α (MIP-1α) in monocytes with normal state or lipid peroxidation injury. Methods The cultured monocytes were maintained in medium and were divided into 6 groups: Control group; Group A (The monocytes were exposed to diamide alone); Group B (The monocytes were pretreated with PPARα agonist Wy14643 at different concentrations); Group C (The monocytes were exposed to diamide after pretreated with Wy14643 at different concentrations); Group D (The monocytes were pretreated with Wy14643 for the different time); Group E (The monocytes were exposed to diamide after pretreated with Wy14643 for different time). After pretreatment, the monocytes were harvested, and the MIP-1α protein was detected by immunocytochemistry. Results Immunocytochemistry analysis showed that exposure of monocytes to diamide alone resulted in a 1.26-fold increase of the levels of MIP-1α protein expression as compared to the control (P<0.01) and MIP-1α protein expression could be significantly induced by PPARα agonist Wy14643 alone or in combination with diamide in monocytes (P<0.01), but reduced when time being. Conclusion PPARα might be involved in the regulation of the expression of MIP-1α protein, suggesting it may participate in the atherogenesis.

关 键 词：PPARΑ MIP-1Α 表达 联胺 人单核细胞 激动剂 蛋白 过氧化物酶体 作用时间 增殖 

分 类 号：R543.5[医药卫生—心血管疾病] R541.4[医药卫生—内科学]