RDP1258对大鼠脾细胞增殖及HO-1活性的抑制作用  

Inhibitory effects of HLA-derived peptide RDP1258 on proliferation and heme oxygenase-1 activity of rat splenocytes

作　　者：易善红[1] 宋波[1] 汪泽厚[1] 张艮甫[1] 崔志刚[1] 

机构地区：[1]第三军医大学附属新桥医院泌尿外科,重庆400037

出　　处：《细胞与分子免疫学杂志》2004年第5期632-634,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家新药基金资助项目 (No.1 999A0 0 2 )

摘　　要：目的 :观察一种新型HLA衍生肽 (RDP12 5 8)的免疫抑制作用 ,并探讨其机制。方法 :用化学方法合成一种HLA衍生肽(RDP12 5 8) ,以3 H TdR掺入法观察其在体外对大鼠脾细胞增殖的影响 ;以酶化学法观察其对脾细胞血红素氧合酶 1(hemeoxygenase 1,HO 1)活性的影响。结果 :RDP12 5 8可显著抑制大鼠脾细胞增殖并以剂量依赖的方式降低HO 1的活性。结论 :RDP12 5 8能够显著抑制丝裂原或同种抗原刺激所致大鼠脾细胞的增殖反应 ,其抑制作用可能是通过降低HOAIM: To observe the immunosuppression effect of a novel HLA-derived peptide RDP1258, and to explore its mechanisms. METHODS: RDP1258 was synthesized chemically. The effect of the peptide on alloreactive proliferation of rat splenocytes was observed by 3H-TdR incorporation method. The heme oxygenase-1(HO-1) activity was analyzed by the enzyme assay. RESULTS: The results showed that the synthetic HLA-derived peptide could obviously inhibit the proliferation of rat splenocytes and HO-1 activty in a dose-dependment manner in vitro. CONCLUSION: HO-1 may participate in the inhibitory effect of RDP1258 on the proliferation of rat splenocytes induced by mitogen or isoantigen.

关 键 词：脾细胞 大鼠 增殖 HO-1 观察 HLA衍生肽 抑制作用 RDP 活性 化学方法 

分 类 号：R392[医药卫生—免疫学] R541.4[医药卫生—基础医学]