尿游离皮质醇化学发光免疫分析  

A New Method of Chemiluminescent Immunoassay for Urinary Free Cortisol Determination

作　　者：刘金玲[1] 马堪悦[1] 李江[1] 李聪新[1] 高硕[1] 

机构地区：[1]天津医科大学总医院特检中心,300052

出　　处：《放射免疫学杂志》2004年第5期409-411,共3页Journal of Radioimmanology

摘　　要：目的 :应用IMMULITE化学发光系统测定尿游离皮质醇并进行评价。方法 :尿液以二氯甲烷抽提后行化学发光免疫分析。结果 :抽提回收率为 (92 7～ 10 5 33) % ,平均为 97 74 % ;稀释试验O/E比值为 87 9～ 10 7 9,平均为 10 0 76 % ;批内变异系数分别为 9 4 %、3 7%、8 1% ,平均 7 1% ,批间变异系数为 7 7%、5 7%、9 7% ,平均 7 7% ;4 2份样本结果分析本法与RIA相关性好r =0 .90 5 (P <0 .0 1) ;正常人参考范围(17 34～ 116 0 2 ) μg/2 4h。结论 :本法具有良好的准确性、健全性和重复性 ,适用于临床常规测定。Objective To develop a new method for measurement of urinary free cortisol with the IMMULITE Automated Chemiluminisent System. Methods Urinary samples were extracted with dichloromentane and the extract was detected for free cortisol with CCIA. Results The recovery rates of extraction were 92.7%～105.3% (mean 97.74%), O/E% of dilution assay were 87.9%～107.9% (mean 100.76%); Intra-and inter-assay coefficients of variation were 9.4%, 3.7%, 8.1% (mean 7.1%) and 7.7%, 5.7%, 9.7% (mean 7.7%) respectively. Results from 42 samples tested with both CLISA and RIA showed good correlation (r=0.905, P<0.01), The normal reference range of urinary free cortisol ws 17.34～116.02μg/24h. Conclusion This method showed good accuracy, validity and reproducibility and was fit for clinical use.

关 键 词：尿游离皮质醇 化学发光免疫分析 批间变异 正常人 参考范围 RIA 临床 光系统 抽提 重复性 

分 类 号：R446[医药卫生—诊断学] R544.1[医药卫生—临床医学]