鸡卵清蛋白基因调控序列指导人促红细胞生成素转基因鸡的制备  

THE PRODUCTION OF TRANSGENIC CHICKEN OF HEPO DIRECTED BY THE CHICKEN OVALBUMIN REGULATORY ELEMENTS

作　　者：杜立新[1] 赵英会[2] 张念华[3] 

机构地区：[1]中国农业科学院畜牧研究所,北京100094 [2]山东农业大学动物科技学院,山东泰安271018 [3]泰山医学院,山东泰安271000

出　　处：《山东农业大学学报（自然科学版）》2004年第3期321-324,共4页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：山东自然科学基金资助项目 (Z990D0 3)

摘　　要：本研究将鸡卵清蛋白 5!@端调控区调控人促红细胞生成素的pOV -hEPO特异表达载体用脂质体包埋 ,和供体细胞融合后 ,显微注射入 4 5 0枚受体胚盘 ,采用代用蛋壳法培养制备转基因鸡 ,PCR和斑点杂交检测外源基因已整合入早期鸡胚胎生殖系统 。The expression vector pOV-hEPO of human erythropoietin directed by the chicken ovalbumin regulatory elements was embedded in liposome. The donor cells suspended from stage X blastoderm and plasmid pOV-hEPO were co-cultured. Then the co-culture was microinjected into recipient blastoderm at the same stage of development. The recipient embryos were cultured with surrogate eggshell. PCR and Dot blot analysis indicated than hEPO gene are integrated into the genital gland in early embryos. The results irdicate that it is possible for the production of chicken ovalbumin bioreactor using Lipofection-mediated exogenous gene directed by the chicken ovalbumin regulatory elements.

关 键 词：促红细胞生成素 卵清蛋白 基因调控 EPO 脂质体 受体 早期 转基因鸡 代用蛋壳 调控区 

分 类 号：S831[农业科学—畜牧学] R392[农业科学—畜牧兽医]