Involvement of NO in fungal elicitor-induced activation of PAL and stimulation of taxol synthesis in Taxus chinensis suspension cells  

作　　者：XUMaojun DONGJufang ZHUMuyuan 

机构地区：[1]CollegeofLifeSciences,ZhejiangUniversity,Hangzhon310012,China [2]CollegeofFoodSciencesandBiotechnologyandEnvironmentalEngineering,HangzhouUniversityofCommerce,Hangzhou310035,China

出　　处：《Chinese Science Bulletin》2004年第10期1038-1043,共6页

摘　　要：Elicitor prepared from the cell walls of Peni-cillium citrinum induces multiple responses of Taxuschinensis cells, including nitric oxide (NO) generation, se-quentially followed by the activation of PAL and synthesis oftaxol. NO scavenger cPITO and nitric oxide synthase (NOS)inhibitor PBITU prevent the latter two reactions, all of whichare triggered in the absence of elicitor by NO donor sodiumnitroprusside (SNP). The elicitor-induced NO release ofTaxus chinensis suspension cells is strongly inhibited byPBITU. These results demonstrate a causal relationship be-tween NO generation and the latter two reactions of Taxus chinensis cells to the elicitor, and also indicate that NO, pro-duced via NOS in Taxus chinensis cells treated with fungalelicitor, might act as an essential signaling molecule for trig-gering the activation of PAL and synthesis of taxol.Elicitor prepared from the cell walls of Peni-cillium citrinum induces multiple responses of Taxuschinensis cells, including nitric oxide (NO) generation, se-quentially followed by the activation of PAL and synthesis oftaxol. NO scavenger cPITO and nitric oxide synthase (NOS)inhibitor PBITU prevent the latter two reactions, all of whichare triggered in the absence of elicitor by NO donor sodiumnitroprusside (SNP). The elicitor-induced NO release ofTaxus chinensis suspension cells is strongly inhibited byPBITU. Thesc results demonstrate a causal reationship be-tween NO generation and the latter two reactions of Taxuschinensis cells to the elicitor, and also indicate that NO, pro-duced via NOS in Tasus chinensis cells treated with fungalelicitor might act as an essential signaling molecule for trig-gering the activation of PAL and synthesis of taxol.

关 键 词：一氧化氮 PAL 中国红豆杉细胞 真菌诱导 红豆杉醇 抗癌药物 

分 类 号：Q947[生物学—植物学]