益骨胶囊含药血清对SD大鼠成骨细胞分泌NO、NOS的影响  

Effect of Yigu Capsule on NO and NOS of Rat Osteoblasts in Vitro

作　　者：张荣华[1] 王廷春[1] 朱晓峰[2] 蔡宇[1] 彭柯萍[1] 郑仕富[2] 

机构地区：[1]暨南大学药学院,广州510632 [2]暨南大学附属第一医院,广州510632

出　　处：《中药材》2004年第8期593-596,共4页Journal of Chinese Medicinal Materials

基　　金：广东省自然科学基金(No990 475 );广州市科技攻关计划重点项目( 2 0 0 2Z3 E5 0 71);广东省中医药局科研基金(No10 3 0 42 )资助项目

摘　　要：目的 :观察益骨胶囊含药血清对体外培养的大鼠成骨细胞 (OB)表达NO、NOS的影响。方法 :(1)将 4 0只 12月龄SD大鼠分为益骨胶囊组 (高、中、低剂量组 )和生理盐水对照组 ,制备含药血清和对照血清 ;确定最佳灌胃剂量组和最佳血清添加浓度。 (2 )分离、培养新生SD大鼠的颅骨成骨细胞 ,传至第 3代后分为 2组 (含药血清组和空白血清对照组 ) ,分别在培养 2 4h、4 8h和 72h后用RT PCR和试剂盒检测OB表达NOSmRNA的量及其活性变化和OB分泌NO的量。结果 :益骨胶囊含药血清组在 2 4h、4 8h和 72h时OB表达NOSmRNA的量及其活性和分泌NO的量均明显高于空白血清对照组 (P <0 0 1或P <0 0 5 )。结论 :益骨胶囊含药血清能促进OBNO的分泌和NOS的表达 。Objective:The effects of Yigu Capsule on NO and NOS of osteoblasts were studied.Methods:(1)Forty 12-month old Sprague-daweley female rats were divided into randomly four groups (Yigu Capsule high dose group,medium dose group and low dose group;saline group),the drug-containing serum and control serum were prepared.(2)The new-born Sprague-daweley rat Osteoblasts were cultured with different Yigu Capsule drug-containing serum concentration and at different exposure time.The mRNA levels of eNOS and iNOS were measured by RT-PCR;the activities of eNOS and iNOS were measured by test kit at different exposure time.NO secretion of osteoblasts was observed,too.Results:The mRNA levels and the activities of eNOS and iNOS and NO secretion of osteoblasts were higher than the control groups respectively after 48,72 and 96 hours (P<0.01 or P<0.05). Conclusions: Yigu Capsule drug-containing serum can promote NOS mRNA and NOS activities expressions and NO secretion of osteoblasts.These results may be parts of the mechanism of Yigu Capsule preventing and treating osteoporosis.

关 键 词：含药血清 益骨胶囊 NOS SD大鼠 成骨细胞 分泌 对照组 月龄 活性变化 添加浓度 

分 类 号：R285.5[医药卫生—中药学] R363[医药卫生—中医学]