柿叶黄酮抑制LDL刺激的大鼠血管平滑肌细胞增殖  

Effects of Flavone from Leaves of Diospyros kaki on Rat Vascular Smooth Muscle Cells Proliferation Stimulated by Native Low-density Lipoprotein in Vitro

作　　者：欧阳平[1] 贝伟剑[2] 赖文岩[1] 彭文烈[2] 

机构地区：[1]第一军医大学附属南方医院,广州510515 [2]中山大学生命科学学院,广州510275

出　　处：《中药材》2004年第8期600-602,共3页Journal of Chinese Medicinal Materials

基　　金：广州市科技局科技攻关项目 (资助号 :JB0 0 0 0 0 44 8165 )

摘　　要：目的 :观察柿叶黄酮对低密度脂蛋白 (LDL)刺激时离体大鼠胸主动脉血管平滑肌细胞增殖的影响。方法 :体外培养大鼠主动脉血管平滑肌细胞 ,LDL刺激时 ,应用不同剂量的柿叶黄酮作用 2 4h后 ,采用MTS/PES法确定血管平滑肌细胞的增殖状态。结果 :与对照组相比 ,LDL可以明显刺激大鼠血管平滑肌细胞的增殖 ,柿叶黄酮对LDL刺激下的大鼠血管平滑肌细胞增殖具有明显的抑制作用 (P <0 0 5 ) ,并呈现剂量依赖性。结论Objective: To observe whether rat vascular smooth muscle cells(VSMCs) proliferation induced by native low-density lipoprotein(LDL) is affected by flavone from leaves of Diospyros kaki in vitro. Methods: Rat aortic VSMCs were cultured in vitro and treated with LDL and flavone from leaves of Diospyros kaki, respectively, and were observed in comparison with the control group. The ratio of cell proliferation was determined by non-radioactive MTS/PES assay. Results: Compared with control, flavone from leaves of Diospyros kaki can dose-dependently inhibit LDL-stimulated vascular smooth muscle cells proliferation (P<0.05). Conclusions: The flavone from leaves of Diospyros kaki can inhibit proliferation of rat vascular smooth muscle cells exposed to high levels of native LDL. Flavone from leaves of Diospyros kaki may exert vascular protection by inhibiting vascular smooth muscle cell growth associated with hypercholesterolemia.

关 键 词：LDL 柿叶黄酮 血管平滑肌细胞增殖 离体大鼠 刺激 抑制 不同剂量 体外培养 低密度脂蛋白 胸主动脉 

分 类 号：R543[医药卫生—心血管疾病] R151.2[医药卫生—内科学]