子宫内膜异位症中单核-巨噬细胞分泌VEGF水平的研究  

Study in VEGF Level Secreted by Monocytes Macrophages in Endometriosis

作　　者：尹利荣[1] 王宇全[1] 米淑玲[1] 张淑敏[1] 董凯军[1] 

机构地区：[1]天津医科大学第二医院妇科,300211

出　　处：《天津医药》2004年第9期532-534,共3页Tianjin Medical Journal

基　　金：天津市自然科学基金资助项目 (项目编号 :013613311)

摘　　要：目的 :探讨单核 -巨噬细胞分泌血管内皮生长因子(VEGF)的水平在子宫内膜异位症(内异症)发病机制中的作用。方法 :采用酶联免疫吸附试验 ,检测20例内异症患者 (内异症组Ⅱ期2例 ,Ⅲ期8例 ,Ⅳ期10例 )、30例非内异症患者 (对照组 )外周血单核细胞及腹腔液巨噬细胞培养上清液中VEGF的含量。结果 :内异症组腹腔液巨噬细胞培养上清液中VEGF的含量高于对照组 (P<0.05),而外周血单核细胞培养上清液中VEGF的含量与对照组差别无统计学意义。各期内异症组外周血单核细胞及腹腔液巨噬细胞分泌VEGF的水平差别无统计学意义 ,与AFS -r评分无关。结论 :内异症患者主要通过腹腔液巨噬细胞分泌高水平的VEGF ,促进异位内膜的黏附、种植 ,VEGF的分泌水平与临床分期无关。Objective:The concentration of vascular endothelial growth factor(VEGF)was assessed to define the role of monocyte and macrophage in endometriosis pathogenesis.Methods:The concentrations of VEGF in culture media of peritoneal fluid macrophage and peripheral blood monocyte of20patients with endometriosis(2patients in stageⅡ,8patients in stageⅢ,and10patients in stageⅣ)and other30patients of control group were determined by enzyme-linked immunosorbent assay(ELISA).Results:The VEGF concentration of periˉtoneal fluid macrophage supernatant in endometriosis group is significantly higher than that of control group,with statistical significance(P<0.05),but no statistical difference was found in that of peripheral blood monocyte supernatant between the two groups.The VEGF concentrations in the supernatant of monocyte and macrophage showed no significant difference among different stages in endometriosis,and no correlation with AFS-r score.Conclusion:It was peritoneal fluid macrophage not peripheral blood monocyte which facilitate the ectopic endometrium adhesion,plant and growth via secreting high level of VEGF,and VEGF level is not correlated with AFS-r score.

关 键 词：VEGF 内异症 腹腔液 单核-巨噬细胞 分泌 对照组 外周血单核细胞 高水平 结论 意义 

分 类 号：R711.71[医药卫生—妇产科学] R737[医药卫生—临床医学]