速率法与微板法测定定值血清谷丙转氨酶比较  

Comparison between Kinetic Method and Microtiter Plate Method for the Measurement of Alanine Transaminase Activity in Quality Control Sera

作　　者：张孝山[1] 李研[1] 李忠平[1] 朱秀文[1] 潘彤[1] 

机构地区：[1]天津市血液中心,300110

出　　处：《天津医药》2004年第9期573-575,共3页Tianjin Medical Journal

摘　　要：目的 :比较速率法和微板法测定定值质控血清中谷丙转氨酶 (ALT)的准确性和精密度。方法 :应用方法学研究对2种方法测定结果进行比较 ,并进行统计学分析。结果 :速率法测定批号为296UN和141UE的定值血清的偏差率分别为 -2.82 %和 -0.30 % ,微板法的偏差率分别为 -9.23 %和 -5.41 % ;速率法测定两定值血清的批内不精密度分别为2.29 %和0.81 % ,微板法分别为12.03 %和5.32 % ,2种方法的测定结果差别有统计学意义(P<0.05或P<0.01)。速率法测定两定值血清的日间不精密度为3.86 %和1.13 % ,微板法为23.92 %和19.44 %。结论 :有条件的采供血单位应当使用自动生化分析仪测定ALT。Objective:To compare the accuracy and precision of kinetic method with microtiter plate method(MPM)in measuring alanine transaminase(ALT)activity in quality control sera(QCS).Methods:ALT test results by both methods were compared in methodogical study and in statistical analysis.Results:The meaˉsurement lot number by kinetic method was296UN and141UE,the deviation rates of QCS were-2.82%and-0.30%respectively.The deviation rates by MPM were-9.23%and-5.41%respectively.The measurement imprecision of ALT in QCS in one lot by kinetic method were2.29%and0.81%respectively and those by MPM were12.03%and5.32%respectively.There was a significant difference between both measurement results with a statistical significance of P<0.05or P<0.01.The imprecision of both measurement methods by day were3.86%,1.13%and23.92%,19.44%respectively.Conclusions:In order to determine the ALT activity automatic biochemical analyzer should be used if possible.

关 键 词：微板法 速率法 血清谷丙转氨酶 不精密度 ALT 测定结果 质控血清 定值 结论 UN 

分 类 号：R446[医药卫生—诊断学] R457[医药卫生—临床医学]