抗感染重组合异种骨对犬污染性桡骨缺损的一期植骨修复作用  

EFFECT OF ANTI-INFECTIVE RECONSTITUTED BONE XENOGRAFT AS PRIMARY BONE GRAFTING ON REPAIR OF CONTAMINATED RADIUS DEFECT IN CANINE

作　　者：袁志[1] 胡蕴玉[1] 刘建[1] 孙梁[1] 李丹[1] 廉凯[1] 吕荣[1] 

机构地区：[1]第四军医大学西京医院全军骨科研究所,西安710032

出　　处：《中国修复重建外科杂志》2004年第5期352-355,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：全军 95指令性课题基金资助项目(96L 0 52 )~~

摘　　要：目的　探讨抗感染重组合异种骨 ( anti- infective reconstituted bone xenograft,ARBX)对犬污染性桡骨缺损的一期植骨修复的效果。　方法　在重组合异种骨 ( reconstituted bone xenograft,RBX)基础上 ,结合抗生素局部缓释技术 ,制备具有较强抗感染能力和较高成骨作用的 ARBX。取成年杂种犬 8只 ,于双侧桡骨中上段制成 15 mm节段性骨缺损 ,在缺损处注入 5× 10 6 CFU / ml金黄色葡萄球菌 1ml,静置 15分钟后 ,于双侧缺损区分别植入 ARBX、RBX,并用钢板固定。术后 6个月对存活的 6只犬进行取材 ,通过解剖学、X线片、组织学及细菌学检查 ,比较 ARBX和 RBX一期植骨修复犬污染性桡骨缺损的效果。　结果　术后 6个月 ,ARBX侧有 5只完全修复 ,1只中央部 3mm缺损未修复 ,均无骨髓炎表现 ,标本细菌培养均为阴性。 RBX侧有 1只部分修复 ,5只未能修复 ,残留 8～ 13mm缺损 ,均有明显骨髓炎表现 ,标本细菌培养均为阳性。　结论　 ARBX具有较高成骨活性和较强的抗菌能力 。Objective To investigate the effect of anti infective reconstituted bone xenograft (ARBX) as primary grafting on repair of a segmental contaminated defect in canine radius. Methods The contaminated segmental defects of 1.5 cm were made in both radius of 8 canine and 1 ml of staphylococal suspension was injected into the defect region at a concentration of 5×10 6 CFU/ml. ARBX(experimental side) or RBX(control side) was implanted into the two sides of the defects respectively as primary grafting followed by internal fixation. The results were compared between the two grafting materials in repairing the contaminated segmental defect. Results In ARBX side, the defects were repaired completely in 5 cases and partially in 1 case, and there existed no osteomyelitis in all cases; while in RBX side, the defects were repaired partially in 1 case and were not repaired in 5 cases after 6 months of operation, and there existed osteomyelitis in all cases. Conclusion Besides its strong osteoinductive and osteoconductive activity, ARBX is highly antibacterial and can be used as primary grafting in repairing contaminated segmental defects.

关 键 词：骨修复 一期植骨 桡骨缺损 RBX 重组合异种骨 抗感染能力 双侧 标本 存活 成骨作用 

分 类 号：R687[医药卫生—骨科学]