胚胎干细胞诱生的胰岛素分泌细胞移植对糖尿病鼠的治疗作用  

Therapeutic effect of insulin-producing cells induced from embryonic stem cells on diabetic mice

作　　者：刘星霞[1] 缪兵[2] 李府[1] 马秀峰[1] 时庆[1] 沈柏均[1] 

机构地区：[1]山东大学齐鲁医院低温医学研究室,山东省济南市250012 [2]山东大学医学院生理学研究所,山东省济南市250012

出　　处：《世界华人消化杂志》2004年第8期1853-1856,共4页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30271248~~

摘　　要：目的:探讨小鼠胚胎干细胞ES-D3诱导生成的胰岛素分泌细胞(IPCs)皮下移植对糖尿病(DM)模型小鼠的血糖及一般状况的影响. 方法:首先,将ES-D3细胞培养于经处理的鼠胚成纤维细胞滋养层上保持未分化状态扩增,对数生长期时转入无血清含bFGF的DMEM诱导培养液使其进行分化;其次,采用STZ(剂量:200 mg/kg)一次性腹腔注射.Balb/c小鼠制备DM模型;最后,将诱导21 d的IPCs行肩胛部皮下移植给DM小鼠,观察小鼠血糖及一般状况的变化. 结果:ES-D3诱导生成的IPCs在培养基中自我组装形成三维立体细胞簇,可被DTZ染成洋红色.诱导生成的IPCs经两次移植给DM小鼠后5 d,受鼠的血糖水平显著降低,一般状况有所改善,但小鼠的血糖未能降至正常水平;第2 次移植后15 d,血糖水平反弹到与移植前没有差别。结论:ES-D3诱导生成的IPCs皮下移植给DM小鼠,能在一段时间内显著降低受鼠的血糖水平,改善一般状况,对DM小鼠起到一定的治疗作用.AIM: To study the therapeutic effect on diabetic mice of insulin-producing cells induced from embryonic stem cells. METHODS: Firstly, ESCs were induced to differentiate in serum-free DMEM supplemented with bFGF for more than 3 weeks, and DTZ staining was used to identify the induced IPCs; Secondly, experimental diabetes was induced in 6- to 8-week-old male Balb/c mice by a single intraperi-toneal injection (200 mg/kg) of streptozotocin freshly dissolved in 0.1 moL/L of citrate buffer, pH 4.5; Finally, the induced IPCs were harvested at day 21 after differentiation, and grafted subcutaneously in the shoulder of streptozotocin-diabetic mice to observe their glucose-reducing effect. RESULTS: ESCs could be induced to differentiate into IPCs in serum-free DMEM supplemented with bFGF. The induced IPCs were stained crimson red by DTZ, and their transplantation could reduce blood glucose of diabetic mouse significantly. CONCLUSION: ESCs can be induced to differentiate into IPCs in serum-free DMEM supplemented with bFGF, and the induced IPCs transplantation has a certain therapeutic effect on diabetic mice.

关 键 词：小鼠 DM 诱导 皮下移植 胰岛素分泌细胞 血糖水平 治疗作用 一般 状况 结论 

分 类 号：R587.1[医药卫生—内分泌] R285.5[医药卫生—内科学]