大鼠肝部分切除术后肝窦内皮细胞对肝细胞增生的影响  

Effects of liver sinusoidal endothelial cells on proliferation of hepatocytes after partial hepatectomy in rats

作　　者：董晓灵[1] 陈平[1] 朱瑾[1] 熊燕[1] 

机构地区：[1]中国人民解放军第三军医大学西南医院全军肝胆外科研究所,重庆市400038

出　　处：《世界华人消化杂志》2004年第8期1861-1864,共4页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.300700746~~

摘　　要：目的:探讨大鼠肝部分切除术后肝窦血管内皮细胞(LSEC) 对再生肝细胞增生的影响. 方法:建立Wistar大鼠70%的肝切除模型和肝窦血管内皮细胞、肝细胞分离与培养方法,在术后不同的时相点分离残肝的肝细胞和肝窦血管内皮细胞,进行培养.实验分组: 肝部分切除组(PO)和假手术组(SO);肝细胞培养分两组:A组(肝细胞)、B组(肝细胞+LSEC上清液),利用放射免疫法测定肝细胞DNA合成率、免疫组化方法测定肝细胞PCNA表达指数的变化. 结果:在术后的6、24 h,B组的肝细胞的PCNA表达指数较A组显著升高(5.9±0.1 vs 8.9±0.1 P<0.05;38.6±2.6 vs 58.0±3.9 P<0.01).而且B组肝细胞的DNA合成量较A组显著升高(226±18 vs 8.9±0.1 P<0.05;38.6±2.6 vs 58.0±3.9 P<0.011. 结论:体外实验证实肝部分切除术后肝窦内皮细胞上清可以促进肝细胞的增生,促进肝细胞的DNA合成,增强再生肝细胞增生活性.AIM: To explore the effects of liver sinusoidal endothelial cells on the proliferation of the regenerative hepatocytes after partial hepatectomy in normal rats. METHODS: A method to separate and culture hepatocytes and liver sinusoidal endothelial cells (LSEC) was established and the hepacytes and sinusoidal endothelial cells of regenerative liver were separated and cultured at different time points after partial hepaectomy in normal rats. The experiment was divided into two groups: operation group (OP) and sham operation group (SO). The cultivation of hepatocytes was divided into two groups: group A (hepatocytes) and group B (hepatocytes + supernatant of LSEC). The expression index of PCNA in hepatocytes was assayed by immunohistochemistry, and the level of synthesis of DNA in regenerative hepatocytes was assayed by radio-immunity. RESULTS: The expression index of PCNA in hepatocytes cultured in group B increased more significantly than that in group A after partial hepatectomy in 6h and 24 h (5.9 ± 0.1 vs 8.9 ± 0.1 P<0.05; 38.6 ?2.6 vs 58.0 ± 3.9 P<0.01), and so did the level of synthesis of DNA in regenerating hepatocytes cultured in group B than that in group A after partial hepatectomy in 6h and 24 h (226 ± 18 vs 8.9 ± 0.1 P<0.05; 38.6 ± 2.6 vs 58.0 ± 3.9 P<0.01). CONCLUSION: The proliferating ability and the synthesis of DNA in hepatocytes after partial hepatectomy are enhanced by the supernatant from LSEC in vitro.

关 键 词：肝细胞 术后 肝部分切除术 增生 肝窦内皮细胞 鼠肝 DNA合成 培养方法 结论 指数 

分 类 号：R657.3[医药卫生—外科学]