唇癌VEGF和NOS的表达与癌细胞增殖关系的研究  

Correlation of carcinoma cell proliferation and expression of vascular endothelial growth factor and nitric oxide synthase in lip squamous cell carcinoma

作　　者：李晓光[1] 王延秀[2] 王艳春[1] 李毅[3] 杨宪勇[3] 侯刚[4] 王青[4] 

机构地区：[1]泰安市中心医院口腔科,山东泰安271000 [2]泰安市中心医院麻醉科,山东泰安271000 [3]泰安市中心医院中心实验室,山东泰安271000 [4]泰安市中心医院病理科,山东泰安271000

出　　处：《临床口腔医学杂志》2004年第9期544-546,共3页Journal of Clinical Stomatology

摘　　要：目的 :研究唇癌组织血管内皮生长因子 (VEGF)、诱导型一氧化氮合酶 (iNOS)、内皮型一氧化氮合酶(eNOS)的表达与癌细胞增殖的相关性。方法 :应用免疫组化方法检测 42例唇癌手术切除标本VEGF、iNOS、eNOS和增殖细胞核抗原 (PCNA)的分布及表达。结果 :① 2 6例 ( 61.90 % )唇癌组织表达VEGF ,2 8例 ( 66.67% )表达iNOS ,3 1例 ( 73 .81% )表达eNOS ;②VEGF与iNOS的表达具有明显相关性 ,VEGF与eNOS的表达无明显相关性 ;③表达VEGF的唇癌PCNA标记指数 (PLI)明显高于不表达VEGF的唇癌 ;表达iNOS的唇癌PLI明显高于不表达iNOS的唇癌。表达eNOS的唇癌PLI与不表达eNOS的唇癌无显著性差异 (P >0 .0 5 )。结论 :①VEGF与iNOS的表达具有明显相关性 ,说明iNOS在VEGF的生成和发挥作用过程中起重要作用 ,②PLI随着VEGF和iNOS表达的增加而增加 ,说明二者对唇癌细胞增殖具有促进作用。Objective:To study the relation of protein expression of inducible nitric oxide synthase(iNOS), endothelial nitric oxide synthase(eNOS) and Vascular endothelial growth factor (VEGF)as related to lip squamous cell carcinoma proliferation.Method:Lip squamous cell carcinoma tissue specimens were obtained from 42 patients undergoing operation,by using the immunohistochemisry technique, the expression of iNOS, eNOS and VEGF and the expression and distribution of PCNA(proliferating ce11 unclear angigen,PCNA) were studied.Result:①Positive VEGF and iNOS and eNOS immunostaining were detected in 26(61.90%),28(66.67%)and 31(73.81%)patients with lip squamous cell carcinoma respectively.②Expression of VEGF bore a significant positive relation to iNOS, but not eNOS. ③PLI(PCNA labelling index) in VEGF and iNOS positive case was significantly higher than that in VEGF and iNOS negative cases. No difference PLI level was noted in both eNOS positive and negative cases.Conclusion:①VEGF and iNOS bear a significant positive correlationship in lip squamous cell carcinoma, showing that iNOS ,rather than eNOS, plays an important role in VEGF production.②PLI increased with the increment of VEGF and iNOS expression. VEGF and iNOS are known to enhance lip squamous cell carcinoma cell proliferation.

关 键 词：血管内皮生长因子 一氧化氮合酶 唇癌 细胞增殖 

分 类 号：Q786[生物学—分子生物学]