Nd:YAP激光照射犬离体支气管壁生物效应的实验研究  

Experimental Study of Biological Effects on Canine Bronchia by Nd:YAP Laser

作　　者：秦梅香[1] 包晓青[1] 袁霞雯[1] 张慧国[1] 张美珏[1] 施虹敏[1] 

机构地区：[1]上海第二医科大学附属仁济医院上海市激光医学研究中心,上海200001

出　　处：《应用激光》2004年第4期243-246,共4页Applied Laser

摘　　要：目的:研究Nd:YAP激光不同剂量的组合对犬离体支气管壁照射后生物效应的差异。方法:以犬作为实验对象, 以不同功率、不同照射时间照射离体支气管壁,通过肉眼、光镜、电镜观察不同激光参数的生物学作用。结果:Nd:YAP激光 光斑直径3mm,作用于厚度2mm的离体支气管壁,以热凝固效应为主,也有一定的消融作用。全层凝固变性的剂量为10w、 3s。结论:Nd:YAP激光的组织消融、热凝固作用与平均功率、照射时间成正相关。光斑直径3mm,作厚度2mm的支气管壁 凝固、消融时,以功率10w、3s以下的剂量较为安全,可减少穿孔的机率。Objective: To observe the difference of biological effects on canine bronchia by Nd:YAP laser with different power and irradiation time. Method: The canine bronchia were irradiated with different power and different irradiation time. We observed the effects of ablation and thermal coagulation of different laser settings. We evaluated the damage with naked eyes, microscope and electroscope. Result:The thermal coagulation is primary and ablation is secondary. The dose of perforation is 10w, 3s. The diameter of facula is 3mm. Conclusion: (1) The thermal coagulation is primary and ablation is secondary of Nd:YAP laser on canine bronchia. The ablation and thermal coagulation effect of Nd:YAP laser on canine bronchia is relevant to mean power and irradiation time. (2) Safety dose is less than lOw, 3s. The diameter of facula is 3mm.

关 键 词：支气管 离体 ND:YAP激光 消融 生物效应 实验研究 剂量 光照射 变性 照射时间 

分 类 号：TN248[电子电信—物理电子学] R322.62[医药卫生—人体解剖和组织胚胎学]