HPLC-MS法测定人血浆中米氮平的浓度  

Determination of mirtazapine in human plasma by HPLC-MS

作　　者：周红华[1] 马仁玲[1] 仇益群[2] 濮社班[3] 黄仲义[2] 

机构地区：[1]中国药科大学分析测试中心,南京210009 [2]上海市静安区中心医院临床药理科,上海200040 [3]中国药科大学中药学院生药研究室,南京210009

出　　处：《中国临床药学杂志》2004年第5期281-284,共4页Chinese Journal of Clinical Pharmacy

摘　　要：目的 :建立一种用高效液相色谱 -电喷雾离子化质谱 (HPLC -MS)联用技术测定米氮平血药浓度的方法。方法 :以0 0 1mol·L-1乙酸铵水溶液∶甲醇 (2 7∶73,V/V)为流动相 ,盐酸地尔硫 艹卓 为内标 ,血浆样品经乙酸乙酯萃取后上样 ,经C18柱分离后 ,以质谱为检测器 ,采用选择性离子检测 (SIM)测定人体血浆中米氮平的浓度。结果 :米氮平的线性范围 0 2 0～ 15 0 μg·L-1(r=0 9995 ) ,平均相对回收率在 90 %～ 110 %之间 ,日内和日间RSD均 <5 % ,米氮平的最低定量限为 0 2 0 μg·L-1,提取回收率 >85 %。结论 :该方法快速、准确、灵敏 。AIM: To develop a HPLC and electrospray ionization mass spectrometry (HPLC-ESI-MS) method for determination of mirtazapine in human plasma. METHODS: The mobile phase was composed of 0.01 mol·L -1 ammonium acetate-water: methanol(27∶73,V/V) and diltiazem hydrochloride was used as internal standard. Plasma samples were extracted with ethyl acetate and separated by C 18 column. The single quadrapole MS was used as detector. Selected ion monitor(SIM) was used for determination of mirtazapine in human plasma. RESULTS: Standard curve was linear in the range of 0.20 to 150 μg·L -1(r=0.999 5),the relative recovery was between 90%-110%. Relate standard deviation (RSD) of intra-day and inter-day assays were all less than 5%. The minimum quantitative limited was 0.20 μg·L -1. The extraction recovery was more than 85%. CONCLUTION: The method proves to be a stable and reliable method for determination of mirtazapine in human plasma as well as for pharmacokinetic studies.

关 键 词：米氮平 HPLC 人血浆 MS 地尔硫革 人体血浆 药动学研究 结论 灵敏 方法 

分 类 号：R96[医药卫生—药理学]