转染人转化生长因子-β_3基因对瘢痕成纤维细胞Ⅰ、Ⅲ型胶原合成的影响  

Effects of transfected human transforming factor β_3 on type Ⅰand Ⅲ collagen synthesis and fibroblasts from hypertrophic scar

作　　者：朱小波 宋朝骏 张进德 袁启智[2] 黄硕麟[2] 

机构地区：[1]上海市浦东新区慈善医院,200135 [2]上海市第一人民医院

出　　处：《上海医学》2004年第9期677-679,共3页Shanghai Medical Journal

摘　　要：目的　观察转染人转化生长因子 β3 (hTGF β3 )基因对瘢痕成纤维细胞Ⅰ、Ⅲ型胶原合成的影响。方法　通过脂质体介导的方法 ,将人表皮细胞生长因子基因真核表达质粒 (pBK CMV) TGF β3 质粒转染体外培养的增生性瘢痕成纤维细胞 ,采用RT PCR法检测TGF β3 mRNA的表达 ,放射免疫 (RIA)法测定细胞培养上清液中Ⅰ型和Ⅲ型前胶原的浓度。结果　构建的TGF β3 质粒可成功转染成纤维细胞 ,并增强Ⅰ型和Ⅲ型胶原的表达 ,下调Ⅰ /Ⅲ型胶原比值。结论　转染hTGF β3Objective To observe the effects of transforming factor β 3(TGF-β 3) gene transfer on fibroblasts from hypertrophic scar and the type Ⅰand Ⅲ collagen synthesis.Methods Fibroblasts were harvested from hypertrophic scar and then were transferred with pBK-CMV-TGF-β 3 plasmid mediated by Lipofectamine. The TGF-β 3 mRNA expression was assayed by RT-PCR. The type Ⅰand Ⅲ collagen contents in the culture supernatant were determined by RIA.Results pBK-CMV-TGF-β 3 plasmid could be transfected into the fibroblasts successfully. The gene-transfected fibroblasts increased the secretion of type Ⅰand Ⅲ collagen and downregulated the ratio of typeⅠand Ⅲ collagen.Conclusion Human transforming factor β 3(hTGF-β 3) gene can regulate the biological activity of fibroblasts from hypertrophic scar.

关 键 词：TGF-Β3 Ⅲ型胶原 转染 瘢痕成纤维细胞 转化生长因子-Β3 基因 真核表达质粒 RNA 质粒 RT—PCR 

分 类 号：R622[医药卫生—整形外科]