中华芦荟组织培养物的芦荟素含量测定  

Determination of Aloin Content in Callus of Aloe vera var.chinensis

作　　者：王红星[1] 李发启[1] 王太霞[1] 李建军[1] 李俊英[1] 杨相甫[1] 李景原[1] 

机构地区：[1]河南师范大学生命科学学院

出　　处：《中药材》2004年第9期627-628,共2页Journal of Chinese Medicinal Materials

摘　　要：以中华芦荟的根、茎、叶为外植体 ,探讨了诱导的愈伤组织与芦荟素积累的关系。分别用薄层色谱法和高效液相色谱法测定。结果表明 ,在MS +NAA 1mg/L + 6 BA 0 5mg/L培养基上 ,以叶为外植体产生的愈伤组织 ,分化程度最高 ,芦荟素含量也高 ( 0 35 % ) ;以茎为外植体产生的愈伤组织 ,芦荟素含量次之 ( 0 0 8% ) ;以根为外植体产生的愈伤组织不含芦荟素。在MS + 2 ,4 D 1mg/L + 6 BA 0 5mg/L培养基上 ,以根、茎、叶为外植体产生的愈伤组织 ,分化程度低 。The relationship between aloin accumulation of Aloe vera var chinensis and the callus cultured by the roots,stems and leaves as explants The aloin content in callus was determined by means of HPLC and TLC The results showed that on the MS medium with NAA 1mg/L+6 BA 0 5mg/L,the differentiation degree of the callus induced from the leaves was in the highest level,meanwhile the callus contained the most aloin. The aloin content was low in the callus from stems. There was no aloin in callus from roots It was also found that on the MS medium with 2,4 D 1 mg/L+6 BA 0 5 mg/L, the callus differentiation was in low level and without aloin,no matter what organs were used

关 键 词：外植体 愈伤组织 中华芦荟 6-BA 组织培养 培养基 NAA 芦荟素 分化程度 含量测定 

分 类 号：R285[医药卫生—中药学] R286.0[医药卫生—中医学]