高效液相色谱法测定飞龙掌血中异茴芹香豆素含量  

Quantification of isopimpinellin in root of Toddalia Asiatica by HPLC

作　　者：郝小燕[1] 曹晓红[2] 梁妍[1] 沈月毛[2] 

机构地区：[1]贵阳医学院药学院,贵州贵阳550004 [2]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550002

出　　处：《中国中药杂志》2004年第8期768-769,共2页China Journal of Chinese Materia Medica

摘　　要：目的 :用高效液相色谱法对苗药飞龙掌血中异茴芹香豆素含量进行测定。方法 :采用HypersilC18柱为分析柱 ,以甲醇 水 (70∶30 )为流动相 ,检测波长 30 6nm。结果 :线性范围 0 .0 0 4 2 0～ 0 .4 2 0 μg ,平均回收率 99.7% ,RSD =2 .8%。结论 :本法简便、准确、重现性好 。Objective: To Determine the content of isopimpinellin in root of Toddalia asiatica. Methods: A HPLC method was set up. Using Hypersil C_(18) column and methanol-water (70∶30) as mobile phase, with the detection wavelength at 306 nm. Result: The linear range of isopimpinellin was 0.004 20～0.420 μg. The average recovery was 99.7% and the RSD 2.8%. Conclusion: The method is simple and accurate, with good reproducibility, and can be used as a quantitative analysis method for isopimpinellin.

关 键 词：高效液相色谱法 香豆素 含量测定 苗药 RSD 检测波长 流动相 C18柱 平均回收率 

分 类 号：R284.1[医药卫生—中药学]