阴道毛滴虫衰老相关基因Tv-Sir2-like克隆与序列分析  

Cloning and Sequence Analysis of Trichomonas vaginalis Aging-related Gene Tv-Sir2-like

作　　者：张可浩[1] 傅玉才[1] 章家新[1] 刘红[1] 

机构地区：[1]汕头大学医学院寄生虫学教研室,广东汕头515041

出　　处：《汕头大学医学院学报》2004年第3期132-135,共4页Journal of Shantou University Medical College

基　　金：汕头大学研究与发展基金项目 (L0 0 0 1 3)

摘　　要：目的 :克隆单细胞模式生物阴道毛滴虫 (Tv)Sir2基因 ,以便探讨其功能。方法 :构建TvcDNA表达文库 ,筛选出Tv_Sir2_like基因 ,并对该基因进行序列分析。结果 :成功克隆Tv_Sir2_like基因。测序及序列分析显示 ,Tv_Sir2_like基因开放读码框长 1116bp ,编码 3 71个氨基酸残基的蛋白质。该氨基酸序列与SIR2的同源性最高 ,并具有SIR2 3个特征性的高度保守结构域。结论 :推测Tv_Sir2_like是Sir2的同源基因 ,其表达产物可能参与Tv转录沉默 。Objective:To clone and characterize Trichomonas vaginalis(Tv)Sir2-likegene.Method:The cDNA library of Tv was constructed and cDNA clones were isolated.One of the cDNA clones,which showed homology with yeast Sir2,was further analyzed.Results:The Tv-Sir2-likegene was cloned successfully,and the sequence analysis revealed that Tv-Sir2-likecDNA contained an open reading frame of 1?116 bp.The deduced amino acid sequence had 371 residues and was homologous to SIR2.The sequence analysis demonstrated that this protein shared all the three characteristic conserved core domains with yeast SIR2 and its homologues.Conclusion:Tv-Sir2-like is a homologous gene of Sir2.Tv-Sir2-likemay participate in transcription silencing and in the cell cycle regulation of Tv.

关 键 词：阴道毛滴虫 E基因 克隆 序列分析 衰老相关基因 CDNA表达文库 细胞周期 结构域 氨基酸序列 转录 

分 类 号：R373[医药卫生—病原生物学] R711.31[医药卫生—基础医学]