乙型病毒性肝炎血清IL-1、IL-8和TNF-α检测及意义  

Detection of IL-1, IL-8 and TNF-α in serum samples from patients with viral hepatitis B and its significance

作　　者：陈瑞烈[1] 王文锋[1] 周震[1] 刘敏[1] 陈造基 林立民[1] 

机构地区：[1]广东省汕头市第二人民医院,汕头515011

出　　处：《传染病信息》2004年第3期114-115,共2页Infectious Disease Information

摘　　要：目的探讨各型乙型病毒性肝炎患者血清白细胞介素-1(IL-1)、白细胞介素-8(IL-8)和肿瘤坏死因子-α(TNF-α)水平的变化及其临床意义。方法采用双抗体夹心 ELISA 法检测25例急性肝炎,29例慢性肝炎,23例重型肝炎和21例肝硬化患者血清 IL-1、IL-8和 TNF-α水平,并与20例正常人进行比较。结果各型乙型肝炎患者血清 IL-1、IL-8、TNF-α平均明显高于正常人(P<0.01);各型乙型肝炎血清 IL-1、IL-8和 TNF-α水平升高,以重型肝炎最明显(P<0.05);IL-1、IL-8和 TNF-α水平与 ALT、TBIL、PT 呈正相关。结论血清 IL-1、IL-8和 TNF-α参与各型乙型肝炎的免疫病理损伤,血清中 IL-1、IL-8、TNF-α水平可作为判断肝脏炎症活动程度、监测病情和判断预后的指标。Objective To investigate the changes of serum levels of IL-1,IL-8 and TNF-α in patients with various types of viral hepatitis B and explore the clinical significance of the detection.Methods The serum levels of IL-1,IL-8 and TNF-α were determined in 25 patients with acute hepatitis,29 with chronic hepatitis,23 with severe hepatitis,21 with liver cirrhosis and 20 healthy individuals with double-antibody sandwich ELISA and then compared.Results The serum levels of IL-1,IL-8 and TNF-α were significantly higher in all the patients than those in the healthy individuals(P< 0.01).The highest serum levels of IL-1,IL-8 and TNF-α were found in the patients with severe hepatitis(P<0.05).The serum levels of IL-1,IL-8 and TNF-α were positively related to those of ALT,TBIL and PT.Conclusions IL-1,IL-8 and TNF-α play an important role in process of immunopathogenic injury of the liver in various types of hepatitis B.Detection of serum IL-1,IL-8 and TNF-α is useful for assessing the degree of liver inflammatory reaction,monitoring progression and predicting prognosis of hepatitis.

关 键 词：IL-1 IL-8 TNF-Α 血清 乙型病毒性肝炎 正常人 重型肝炎 乙型肝炎 TBIL 肝脏炎症 

分 类 号：R512[医药卫生—内科学] R575[医药卫生—临床医学]