人类新基因RHBDL8的研究初报  

Studies on a Human Novel Gene RHBDL8

作　　者：朱传炳[1] 朱莹[1] 曾伟奇[1] 王跃群[1] 李永青[1] 袁婺州[1] 吴秀山[1] 

机构地区：[1]湖南师范大学生命科学学院发育生物学与蛋白质化学系,中国长沙410081

出　　处：《湖南师范大学自然科学学报》2004年第3期79-82,共4页Journal of Natural Science of Hunan Normal University

基　　金：国家自然科学基金(30170479;30270644;30213904);湖南省自然科学基金(02JJY4026;0355Y4011);教育部与省教育厅基金(02A028;02B017;03C208)资助项目

摘　　要：运用计算机克隆的方法获得了一个新的人同源基因,经国际基因命名委员会批准命名为Rhomboid,veinlet like8(RHBDL8).该基因位于7号染色体q11.23区域,mRNA全长约1.8kb,3'末端含有ATAAA的加尾信号,编码一段长434个氨基酸的蛋白质.该蛋白质含有一个rhomboid保守区,且高度相似于一个未有研究的小鼠蛋白.其表达在胚胎期(23周)主要集中在大脑、心脏、骨骼肌,而在成体,除脑部依然有大量表达外,上述的其他部位表达量均大大降低.这种在发育过程中的表达变化提示我们该基因可能在这些器官的发育中起到了一定的调控作用.With computer cloning method we cloned a human homologous gene, named Rhomboid, veinkt-like 8 (RHBDL8), which is approved by HUGO Gene Nomenclature Committee. This gene is located in chromosome 7q11.23 and its mRNA is about 1.8 kb with a polya signal ATAAA at its 3' -terminal. RHBDL8 encodes for a protein of 434 amino acids, which contains a rhomboid domain and is highly identical to a mouse protein of unknown function. This gene is expressed mainly in brain, heart, lung and skeletal muscle at embryo stage (23 week), while besides the remained high expression in brain, the expression level in most of the organs decreases greatly at adult stage. This spatio-temporal change in expression pattern suggests that RHBDL8 may be involved in regulation of the development of these organs.

关 键 词：新基因 表达量 et-l 心脏 脑部 小鼠 量表 计算机克隆 同源基因 成体 

分 类 号：Q785[生物学—分子生物学] Q78