LPSp作为乙型肝炎表面抗原佐剂的初步研究  

Primary research on LPSp as adjuvant to HBsAg

作　　者：王启辉[1] 冷静[1] 王健[1] 郑禹[1] 

机构地区：[1]广西医科大学微生物学与免疫学教研室,南宁530021

出　　处：《广西预防医学》2004年第5期257-259,共3页Guangxi Journal of Preventive Medicine

基　　金：广西壮族自治区科技厅资助项目 (桂科攻9817075)

摘　　要：目的研究革兰阴性非致病菌成团泛菌脂多糖 (Pantoeaagglomeranslipopolysaccharide,LPSp)作为佐剂对重组乙型肝炎表面抗原 (HBsAg)诱导小鼠产生抗 -HBs抗体的影响。方法采用纯系 (Balb/c)小鼠作为免疫对象 ,经背部皮下免疫2次 ,ELISA法检测血清乙型肝炎表面抗体(抗 -HBs)滴度。结果HBsAg加LPSp组 ,其抗 -HBs总抗体滴度较单独注射HBsAg明显增高 (P<0.05) ;抗体持续时间可更长。结论LPSp对小鼠抗 -HBs抗体的产生具有增强作用 。Objective To investigate the effect of avirulent Pantoea agglomerans lipopolysaccharide(LPSp)as adjuvants on the production of anti_HBs in mice immunized with recombinant hepatitis B surface antigen(HBsAg).Methods The antigens were injected into the back hypoderm of all the mice(Balb/c breed)for two times.The serum anti_HBs titers were detected by ELISA.Results The anti_HBs polyclonal antibody titers from mice immunized with additional LPSp were higher than those of mice without LPSp(P<0.05).The antibody persistent time was potentially longer in additional LPSp groups.Conclusion LPSp has enhanced efficacy for anti_HBs production in immunized mice.

关 键 词：LPS 抗-HBS 佐剂 抗体 乙型肝炎表面抗原 小鼠 皮下 纯系 免疫 成团泛菌 

分 类 号：R512.62[医药卫生—内科学] R392[医药卫生—临床医学]