分枝杆菌菌种快速荧光初步鉴定方法的研究  

Study on the method of rapid fluorimetry for primitive identification of species of Mycobacterium

作　　者：施旭东[1] 刘正华[1] 吴晓渊[1] 王雷[1] 

机构地区：[1]南京市胸科医院,南京210029

出　　处：《中国防痨杂志》2004年第5期295-297,共3页Chinese Journal of Antituberculosis

摘　　要：目的　使用液体培养基 ,建立一种分枝杆菌 (Mycobacterium)菌种初步鉴定的非放射快速荧光检测法。方法　应用自制的非放射荧光检测管 ,利用水溶性对硝基苯甲酸 (P NitrobenzoicAcid ,PNB)和噻吩 - 2 -羧酸肼 (2 Thiophenecarborylicacidhydrazide ,TCH)对 15株分枝杆菌标准菌株和 78株分枝杆菌临床分离菌株做体外抑菌试验 ,确定其在菌群鉴定试验中的应用浓度。结果　PNB的应用浓度为 10 0 μg/ml ,可有效地区别结核杆菌复合群与非结核分枝杆菌 ;TCH的应用浓度为 2 .5 μg/ml ,可有效地区别结核分枝杆菌和牛分枝杆菌。菌群鉴定结果 7～ 10d可得到报告。结论　本法经济实用 ,并将分枝杆菌菌群鉴定结果报告时间由传统的L J方法的 2 8d缩短为 7～ 10d。Objective To set up a method of Rapid Fluorimetry for identification of group of Mycobacterium by using the liquid culture medium.Methods Examined the drug susceptibility in vitro of 15 strains standard Mycobacterium and 78 strains Mycobacterium from clinical samples to P Nitro benzoic Acid (PNB) and 2 Thiophene carborylic acid hydrazide (TCH) by the examining tube of radioactive Free and rapid fluorimetry anddetermined the concentration of PNB and TCH in testing of identification of group of Mycobacterium.Results 100μg/ml of PNB can identify Mycobacterium tuberculosis complex and non tuberculosis mycobacterium (NTM),2.5μg/ml TCH can identify mycobacterium tuberculosis and Mycobacterium bovis .The test just took 7～10 days.Conclusion This method is economic,practical and the report time was shorten significantly for identification of group of mycobacterium.The report time of the method was 7～10 days.It was 18～21 days shorter than that of the method of L J (28 days).

关 键 词：非结核分枝杆菌 菌群 放射 临床分离菌 结核杆菌 TCH 荧光检测法 区别 结论 经济实用 

分 类 号：R446.5[医药卫生—诊断学]