固体二氧化氯消毒剂杀灭微生物效果与毒性试验研究  

EXPERIMENTAL STUDY ON GERMICIDAL EFFICACY AND TOXICITY OF STABLE CHLORINE DIOXIDE DISINFECTANT

作　　者：孙俊[1] 陈越英[1] 吴小成[1] 周品众[1] 杨明晶[1] 

机构地区：[1]江苏省疾病预防控制中心,南京210009

出　　处：《中国消毒学杂志》2004年第4期296-298,304,共4页Chinese Journal of Disinfection

摘　　要：为了解稳定性固体二氧化氯消毒剂杀菌效果与毒性 ,在实验室进行了定量载体杀菌试验与毒性试验。结果 ,以含二氧化氯 10 0mg/L的消毒液作用 3min ,对布片上金黄色葡萄球菌和大肠杆菌的平均杀灭率为 99.9%以上 ;含二氧化氯 2 5 0mg/L消毒液作用 15min对布片上枯草杆菌黑色变种芽孢的平均杀灭率为 99.97%。含二氧化氯 2 0 0mg/L消毒液作用 15min和 2 0min对瓷碗和竹筷上大肠杆菌的杀灭率均为 10 0 %。毒性试验结果表明 ,该消毒剂对雌性小鼠急性经口LD50 为 2 0 0 0mg/kg体重 ,对雄性小鼠LD50 为 1710mg/kg体重 ,属于低毒级 ;蓄积系数K >5 ,属于弱蓄积毒性 ;该消毒剂试验剂量对小鼠骨髓嗜多染红细胞无致微核作用。该消毒剂于 5 4℃贮存 14d ,二氧化氯下降率为 3.15 %。结论 ,该消毒剂具有良好的杀灭微生物效果 ,性能稳定 ,使用方便 ,毒性低。In order to know the germicidal efficacy and toxicity of a stable solid chlorine dioxide disinfectant, quantitative carrier germicidal test and toxicity test were carried out in laboratory. The results showed that the disinfectant solution containing chlorine dioxide 100 mg/L with a 3 min contact time killed over 99.9% of Staphylococcus aureus and Escherichia coli on cloth strips in average, the solution containing chlorine dioxide 250 mg/L with a 15 min contact time killed 99.97% of spores of Bacillus subtilis var. niger on cloth strips in average. The solution containing chlorine dioxide 200 mg/L with a 15 and 20 min contact time killed 100% of E. coli on porcelain bowls and chopsticks. The results of toxicity test indicated that the acute oral LD 50 of this disinfectant was 2000 mg/kg body weight in female mice and 1710 mg/kg body weight in male mice, so it belongs to low toxic grade. Its cumulative coefficient K was>5, belonging to weak cumulative toxicity. The test dosage of this disinfectant did not cause micronuclei in polychromatic erythrocytes of mouse bone marrow. Its chlorine dioxide content decreased by 3.15% after storing at 54℃ for 14 days. Conclusion: This disinfectant has good germicidal efficacy and low toxicity and is stable and convenient for use.

关 键 词：二氧化氯 金黄色葡萄球菌 大肠杆菌 枯草杆菌黑色变种芽孢 稳定性 毒性 

分 类 号：R187.2[医药卫生—流行病学]