大鼠坐骨神经损伤后感觉神经元死亡数量的研究  

Quantitative analysis of dead dorsal root ganglion neurons following sciatic nerve injury in adult rats

作　　者：尹方明[1] 李铁林[1] 段传志[1] 柯以铨[1] 汪求精[1] 郭元星[1] 方兵[1] 

机构地区：[1]第一军医大学珠江医院神经外科,广州510282

出　　处：《中华实验外科杂志》2004年第9期1103-1104,共2页Chinese Journal of Experimental Surgery

摘　　要：目的 研究周围神经损伤后,脊神经节感觉神经元的死亡数量。方法 先计算正常SD大鼠两侧的脊神经节感觉神经元胞体数量是否对称,再切断并原位吻合右侧大鼠坐骨神经,左侧不作任何处理,于术后不同时间取L4-L6脊神经节作苏木素-伊红(HE)染色,计算脊神经节感觉神经元胞体数量的变化。结果 正常SD大鼠两侧的脊神经节感觉神经元胞体数量呈对称分布;右侧坐骨神经损伤后,其脊神经节感觉神经元胞体数量较左侧减少:30 d后,脊神经节细胞的死亡比率达30％,神经元死亡高峰在神经损伤后的第2周内。结论大鼠坐骨神经损伤后,脊神经节感觉神经元的胞体有死亡,其死亡具有一定的特征。Objective To analyze quantitatively the dead dorsal root ganglion neurons following peripheral nerve injury in adult rats. Methods The bilateral number of L4-L6 dorsal root ganglion neurons in normal adult SD rat was counted up. Then the right sciatic nerve of SD rat was cut and anastomo-sized. At different time points after operation, the L4-L6 dorsal root ganglia stained with HE was removed and the number of dorsal root ganglion neurons was counted. Results The bilateral number of L4-L6 dorsal root ganglion neurons in normal adult SD rat were symmetry, but the number of right dorsal root ganglion neurons following peripheral nerve injury was reduced. 30 days later, the rate of dead ganglion neurons was 30% .The dead ganglion neurons peaked during the second week of peripheral nerve injury. Conclusion Peripheral nerve injury could induce ganglion neuron death and the neuron death demonstrated itself character.

关 键 词：感觉神经元 脊神经节 坐骨神经损伤 SD大鼠 正常 左侧 死亡 数量 变化 

分 类 号：R651.3[医药卫生—外科学] R338[医药卫生—临床医学]