高压氧对急性下肢缺血再灌注大鼠血小板膜糖蛋白CD31、CD61和CD62p表达的影响  

Effects of hyperbaric oxygen on the expression of platelet membrane glycoprotein-CD31, CD61 and CD62p in rats after acute ischemia reperfusion of hind limb

作　　者：赵立明[1] 原标[2] 高春锦[1] 王国忠[1] 葛环[1] 夏成青[3] 齐曼[3] 

机构地区：[1]首都医科大学附属北京朝阳医院高压氧科,北京100020 [2]首都医科大学附属北京朝阳医院血管外科,北京100020 [3]首都医科大学附属北京朝阳医院病理科,北京100020

出　　处：《中华航海医学与高气压医学杂志》2004年第3期146-148,共3页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的　探讨高压氧 (HBO)对急性下肢缺血再灌注大鼠血小板膜糖蛋白 CD31、CD6 1和CD6 2 p表达的影响。方法　大鼠随机分为下肢缺血再灌注组 (再灌注组 )、下肢缺血再灌注 +HBO处理组 (HBO组 )、假手术组。夹闭大鼠腹主动脉 ,建立急性下肢缺血再灌注大鼠模型。观察急性下肢缺血再灌注大鼠经 HBO治疗后大鼠的血小板膜糖蛋白 CD31、CD6 1和 CD6 2 p表达的变化。结果　再灌注组大鼠血小板膜糖蛋白 CD31和 CD6 2 p表达均高于假手术组 ,差异具有显著性 (P<0 .0 5 ) ,而 HBO组 CD31和 CD6 2 p表达均低于再灌注组 ,差异具有显著性 (P<0 .0 5 )。假手术组、再灌注组和 HBO组 3组之间CD6 1表达差异无显著性。结论　急性下肢缺血再灌注可以导致大鼠血小板膜糖蛋白 CD31和 CD6 2 p的表达量增加 ,而早期 HBO治疗可有效抑制这些血小板膜糖蛋白的表达 ,可能有助于减轻缺血再灌注损伤。Objective To investigate the effects of hyperbaric oxygen (HBO) on the expression of platelet membrane glycoprotein CD31,CD61 and CD62p after acute ischemia reperfusion of hind limb. Methods The rats were randomly divided into 3 groups: the hind limb ischemia reperfusion group (IR group); the hind limb ischemia reperfusion plus hyperbaric oxygen group (HBO group); and sham operation group. An animal model of acute hind limb ischemia reperfusion was developed by clamping abdominal aorta of rats. Changes in the expression of CD31, CD61 and CD62p of platelet membrane in rats were observed following HBO treatment. Results The expression of CD31 and CD62p of platelet membrane in the IR group was significantly higher than that of the sham operation group ( P <0 05), while the expression of CD31 and CD62p in HBO group was significantly lower than that of the IR group ( P <0 05). There were no significant changes in the expression of CD61 among the IR group, the HBO group and the sham operation group. Conclusions Acute hind limb ischemia reperfusion can increase the expression of CD31 and CD62p of platelet membrane in rats, and early HBO treatment can inhibit effectively the expression of CD31 and CD62p, which may help to relieve reperfusion injury.

关 键 词：再灌注 下肢缺血 CD62p CD31 血小板膜糖蛋白 大鼠 急性 表达量 表达差异 显著性 

分 类 号：R459.6[医药卫生—治疗学]