黄精多糖对糖尿病鼠脑组织糖基化终产物受体mRNA表达的影响  

Effect of Polygona-polysaccharose on Expression of RAGE mRNA in Brain of Diabetic Rats

作　　者：吴燊荣[1] 李友元[2] 邓洪波[2] 肖洒 王蓉[2] 

机构地区：[1]中南大学湘雅医学院附属海口医院老年病科,海口市570208 [2]中南大学湘雅医学院附属第二医院老年病科,长沙市410011 [3]海口市中医药学校,海口市570208

出　　处：《中国药房》2004年第10期596-598,共3页China Pharmacy

基　　金：海南省自然科学基金资助项目 (30223)

摘　　要：目的 :探讨黄精多糖对糖尿病鼠脑组织糖基化终产物受体mRNA(RAGEmRNA )表达的调节作用。方法 :小鼠腹腔注射链脲佐菌素建立动物模型 ,采用逆转录聚合酶链反应法测定各组动物脑组织中RAGEmRNA的表达。结果 :糖尿病模型组脑组织RAGEmRNA的表达增加 ,其RAGE/β-actin值明显高于对照组 (P<0 01)。应用黄精多糖治疗后 ,治疗组脑组织RAGEmR NA表达较模型组降低 ,其RAGE/β-actin值显著低于模型组 (P<0 05)。结论 :黄精多糖能抑制糖尿病鼠脑组织RAGEmRNA的表达 ,对高血糖及糖基化终产物造成的脑组织损伤具有保护作用。OBJECTIVE:To study the regulative function of polygona-polysaccharose on the expression of RAGE mRNA in diabetic rats’brain.METHODS:Diabetic rat model was made by intraperitoneal injection of streptozotocin.RT-PCR method was used to examine the expression of RAGE mRNA in rats’brain.RESULTS:The expression of RAGE mRNA in brain tissue of diabetic rats was increased.The value of RAGE/β-actin in diabetic rats was significantly higher than that in the control group(P<0.01).After treatment with polygona-polysaccharose,the expression of RAGE mRNA in brain tissue was decreased in the treated group.The RAGE/β-actin value in the treated group was significantly lower than that in the model group(P<0.05).CONCLUSION:Polygona-polysaccharose may decrease the expression of RAGE mRNA in the brain tissue in diabetic rats and has protective effect on the tissue damage caused by hyperglycemia.

关 键 词：脑组织损伤 黄精多糖 表达 糖尿病 糖基化终产物 受体MRNA 治疗后 应法 结论 目的 

分 类 号：R587[医药卫生—内分泌] R285.5[医药卫生—内科学]