高效液相色谱法测定胃乐胶囊中芍药苷的含量  

Content Determination of Paeoniflorin in Weile Capsule with HPLC

作　　者：李锦绣[1] 时霄霄 王丰云[3] 

机构地区：[1]山东省滨州市人民医院,滨州市256610 [2]山东省邹平县中医院,邹平县256200 [3]山东省邹平县人民医院,邹平县256200

出　　处：《中国药房》2004年第10期626-627,共2页China Pharmacy

摘　　要：目的 :建立以高效液相色谱法测定胃乐胶囊中芍药苷含量的方法。方法 :色谱柱为HypersilC18,流动相为乙腈 -0 1 %磷酸 (18∶82) ,检测波长为230nm。结果 :芍药苷进样量在0 25μg～2 5μg范围内与吸收峰面积值呈良好的线性关系 ,平均加样回收率为97 9 %(RSD=1 09 %,n=5)。结论 :本法测定结果准确、重现性好。OBJECTIVE:To establish HPLC method for determination of the content of paeoniflorin in Weile capsules.M_ ETHODS:Paeoniflorin was separated on Hypersil C 18 column by using acetonitrile-0.1%phosphoric acid(18∶32)as mobile phase and230nm as UV detection wavelength.RESULTS:There was a good linear relationship between the concentration of paeoniflorin and absorption area value in range of0.25μg～2.5μg.The average recovery was97.9%(RSD=1.09%,n=5). CONCLUSION:The method is highly reproducible and accurate.

关 键 词：高效液相色谱法 胃乐胶囊 芍药苷 含量 

分 类 号：R927.2[医药卫生—药学] R944.5