水中贾第鞭毛虫和隐孢子虫检测方法的探讨  

Detection of Giardia and cryptosporidinm in water

作　　者：余贵英[1] 蔡承铿[1] 刘汝青[1] 余淑苑[2] 陈沛棠 陈成章[1] 

机构地区：[1]中山大学公共卫生学院,广东510080 [2]深圳市疾病控制中心,广东518020 [3]澳门自来水有限公司

出　　处：《中国卫生检验杂志》2004年第5期549-550,共2页Chinese Journal of Health Laboratory Technology

摘　　要：本研究参考EPA1623的三个关键步骤:原虫过滤浓缩→分离→染色鉴定,对原虫的检测方法在过滤、浓缩和染色等过程中进行了改进,并用膜过滤-梯度分离-Giem Sa染色、膜过滤-IMS-GiemSa染色及囊过滤-IMS-IFA(EPA1623)三种检测方法对原虫的染色特征,形态结构和回收率的检测进行了方法学的探讨和比较分析。结果显示:三种检测方法G的回收率均在EPA规定的质控范围内,而膜-梯度分离-Giemsa和膜-IMS-Giemsa的C的回收率略低于EPA质控范围。但将EPA方法中的滤囊过滤改成滤膜过滤,免疫荧光染色改成GiemSa染色,能减少成本费用约60%-90%,而且实验条件要求较低,可以用于自来水出厂水和污水(不经膜过滤)中原虫的筛选。In this study,three methods according to EPA1623 were used for detection of Giardia and cryptosporidium in water.The results showed that the r ecovery obtained by the 3 methods was within the quality - control range. The chang e of filtering cyst into filtering membrane and immunofluorescent staining into GiemSa staining can reduce cost for 60 - 90% . The method can be widely used for det ection of Giardia and cryptosporidium in water.

关 键 词：GIEMSA染色 质控 原虫 检测方法 隐孢子虫 免疫荧光染色 IMS EPA 关键步骤 分离 

分 类 号：R123[医药卫生—环境卫生学] S858[医药卫生—公共卫生与预防医学]