RP-HPLC法测定枇杷叶中熊果酸和齐墩果酸的含量  

Determination of Ursolic Acid and Oleanolic Acid in Leaf of Eriobotrya japonica by RP-HPLC

作　　者：罗晓清[1] 郭小仪[1] 俞学炜[1] 

机构地区：[1]江苏苏州卫生学校药学教研室,江苏苏州215002

出　　处：《中国野生植物资源》2004年第5期50-51,共2页Chinese Wild Plant Resources

摘　　要：目的 :建立枇杷叶中熊果酸和齐墩果酸的含量测定方法。方法 :采用RP -HPLC法测定 ,色谱柱为ZorbaxODS柱 ,用甲醇 - 1%醋酸水溶液 (88∶12 )为流动相 ,检测波长为 2 15nm。结果 :熊果酸和齐墩果酸的平均回收率分别为 98.1% ,97.3% (n =3) ,RSD分别为 1.78% ,1.93% (n =3)。结论 :本法准确、灵敏、快速 ,可作为枇杷叶药材及其制剂的质量控制方法之一。Objective:To develop a method for the determination of ursolic acid and oleanolic acid in leaf of Eriobotrya japonica. Method:The determination was carried out with RP-HPLC on Zorbax ODS column ( 4.6 mm×250 mm,5 μm) with methol-1%HAc solution (88∶12) as the mobile phase,and detect at a UV wave length of 215 nm. Results:The average recovery was respectively 98.1% and 97.3% (n=3),RSD 1.78% and 1.93% (n=3) for ursolic acid and oleanolic acid. Conclusion:This method is rapid,simple,accurate and good for the determination of ursolic acid and oleanolic acid in leaf of Eriobotrya japonica.

关 键 词：齐墩果酸 熊果酸 枇杷叶 RP-HPLC法 含量测定方法 RP—HPLC法 制剂 平均回收率 药材 醋酸水溶液 

分 类 号：Q599[生物学—生物化学] R284[医药卫生—中药学]