高效液相色谱法测定商陆植物中皂苷甲的含量  

A Quantitative determination of esculentoside A in radix phytolaccae by high performance liquid chromatography

作　　者：邢浩[1] 庞雪[1] 

机构地区：[1]中日友好医院药学部,北京100029

出　　处：《中日友好医院学报》2004年第5期304-306,共3页Journal of China-Japan Friendship Hospital

摘　　要：目的 :建立高效液相色谱法测定商陆植物中皂苷甲的含量。方法 :采用蒸发光散射检测器 ,高效液相色谱法测定含量 ,色谱柱为YWG C18( 4 6mm× 2 5 0mm ,5 μg) ;流动相为水 :甲醇 ( 6 6∶34) ;流速 1 0ml/min ;检测波长 2 0 7nm。结果 :线性范围为 1 0 4 μg～ 9 36 μg(r =0 .9995 ,n =5 ) ,回收率为 10 0 5 % ,RSD为 1 1%。 结论Objective:The quantity of esculentoside A in radix phytolaccae was determined by HPLC method.Methods:A HPLC-ELSD assay was developed .The sample was separated on YWG-C18(4.6 mm×250 mm,5 μm),with H 2O:CH 3OH(66:34)in the flow phase (flow rate at 1.0 ml/min).The determining wavelength was 207nm.Results:The calibration curve was linear over the range of 1.04～9.36μg( r =0.9995, n =5),the recovery rate was 100.5%,RSD was 1.1%.Conclusion:The method can be used to determine the quavtity of esculentoside A in radix phytolaccae.

关 键 词：皂苷 高效液相色谱法 商陆 含量测定 蒸发光散射检测器 RSD 测定含量 植物 流动相 色谱柱 

分 类 号：R284.1[医药卫生—中药学]