Beagle犬体内MN9202的测定及其代谢动力学  

Determination and pharmacokinetics of MN9202 in Beagle dogs

作　　者：杨志福[1] 周四元[1] 杨铁虹[1] 梅其炳[1] 刘莉[1] 

机构地区：[1]第四军医大学基础部药理学教研室,陕西西安710033

出　　处：《第四军医大学学报》2004年第19期1794-1796,共3页Journal of the Fourth Military Medical University

摘　　要：目的 :建立高效液相色谱法测定Beagle犬血浆中 1,4 二氢 2 ,6 二甲基 4 (3’硝基苯基 ) 3,5 吡啶二甲酸甲戊酯(MN92 0 2 )的方法 ,并研究其代谢动力学规律 .方法 :采用正交设计优化色谱分离条件 .Beagle犬给予二氢吡啶类药物MN92 0 2 (0 .335mg/kg ,iv) ,用反相高效液相色谱法分析血浆中原型药物浓度 ,血浆药物浓度 时间数据用 3P97药代动力学软件分析 .结果 :在选定的色谱条件下 ,MN92 0 2和干扰能得到较好的分离 ,其保留时间 <14min .Beagle犬静脉注射给予MN92 0 2后 ,其体内过程符合二室模型 ,T1/ 2 β为 (2 0 0± 32)min ,曲线下面积 (AUC)为 (32 14 7± 95 4 4 ) μg·min/L .结论 :所建立的方法稳定、简便、准确 ,可用于Beagle犬血浆中MN92 0 2的浓度测定 .MN92 0 2在Beagle犬体内代谢迅速 .AIM: To set up a sensitive and rapid high performance liquid chromatography (HPLC) method to determine MN9202 and to study its pharmacokinetics in Beagle dogs. METHODS: The mobile phase and extraction methods were optimized by the orthogonal experimental design. MN9202 was intravenously administered to the Beagle dogs at the dose of 0.335 mg/kg and MN9202 in plasma was detected by HPLC. The pharmacokinetic parameters were calculated by 3P97 software. RESULTS: Under the selected HPLC conditions, the MN9202 was clearly separated from interference and the retention time of MN9202 was less than 14 min. When MN9202 was intravenously administered, the plasma concentration-time curve was fit to two-compartment model. T 1/2 β was (200±32) min. and AUC was (32 147± 9544)μg·min/L. CONCLUSION: The analytical method we set up is simple, sensitive and accurate, and it can be applied to determine the MN9202 in the plasma of Beagle dogs. The speed of elimination of MN9202 is high in Beagle dogs.

关 键 词：MN9202 药物代谢动力学 高效液相色谱 半衰期 正交试验 

分 类 号：R917.101[医药卫生—药物分析学]