用非离子去垢剂抽提获得的小游仆虫皮层细胞骨架的构形  

Cortical Cytoskeleton of Euplotes gracilis (Protozoa, Ciliophora) by Non-Ionic Detergent Extraction

作　　者：朱慧[1] 邹士法 李艺松[1] 倪兵[1] 顾福康[1] 

机构地区：[1]华东师范大学生命科学学院 [2]Department of Molecular Microbiology & Immunology School of Public Health,Johns Hopkins University,Baltimore,MD 21205,U.S.A.

出　　处：《Zoological Research》2004年第5期422-428,共7页动物学研究（英文）

基　　金：国家自然科学基金资助项目 ( 3 0 2 70 160 )

摘　　要：由扫描电镜术显示 ,应用非离子去垢剂抽提获得的小游仆虫 (Euplotesgracilis)皮层细胞骨架是由非纤毛区皮层骨架、纤毛器骨架及其附属纤维等构成的三维结构网架。各类细胞骨架以纤维物质为基本成分组成纤维网、纤维层、纤维束和纤维薄片等不同形态单元。其中 :非纤毛区皮层骨架以表面纤维网和表膜下纤维层为形态单元位于细胞的外周层 ;纤毛器骨架中的口围带骨架、口侧膜骨架、额腹横棘毛骨架按各自的分布图式在皮层内定位 ,成为主要的皮层骨架结构。尽管这些纤毛器骨架显示不同的形态 ,但却具有相同的建构特征 ,即都是由纤毛器的毛基体、纤毛器托架和骨架附属纤维相互联系镶嵌在一起形成的相对独立的结构单元。分析推测 ,游仆虫皮层表面纤维网使细胞表面形成区域化结构 ,它也可能与细胞表面各部分的联系及其细胞与环境的相互作用有关 ;纤毛器骨架中各个纤毛器的毛基体复合结构可能对纤毛器托架和骨架附属纤维等起到微管组织中心的作用。By means of non-ionic detergent extraction and scanning electron microscopy,it was shown that the cortical cytoskeleton is a three-dimension network composed of non-ciliaed-regional skeleton,ciliature skeleton and skeleton-associated fibers in Euplotes gracilis.All kinds of the skeleton are made up of the fibers forming fiber net,layer,band,sheet and other structural units.The non-ciliaed cortical skeleton is composed of the surface fiber net and the sub-pellicular fiber layer located in the cortical periphery.The ciliature skeletons,i.e.adoral zone of membranelles skeleton,paroral membrane skeleton and frontal-ventral-transverse cirri skeleton,are located in the cortex according to their corticotype and form an elementary part of the ventral cytoskeleton.Although these skeletons manifested different appearances,they possessed identical structure,namely a functional unit formed by the kinetosomes,ciliature bracket and skeleton-associated fibers.It was supposed that the fiber net forming the regional structures on the cell surface might play a role in connection of the surface parts and in the interaction between the cell and its environment.In ciliature cytoskeleton,the kinetosomal complex might play a role of microtubule-organizing center in the formation of ciliature bracket and skeleton-associated fibers and etc.

关 键 词：纤毛 皮层细胞 细胞表面 外周 细胞骨架 去垢剂 形态 纤维网 纤维物质 纤维层 

分 类 号：Q952[生物学—动物学]