蓝尾石龙子精子的超微结构  

Ultrastructure of Spermatozoa of Eumeces elegans

作　　者：刘永章[1] 张永普[2] 

机构地区：[1]温州医学院生物学教研室,浙江温州325027 [2]温州师范学院生命与环境科学学院,浙江温州325027

出　　处：《Zoological Research》2004年第5期429-435,共7页动物学研究（英文）

基　　金：温州市科技发展计划项目基金 (S2 0 0 2A0 2 1) ;温州师范学院科研项目基金 ( 2 0 0 3Z18)~~

摘　　要：蓝尾石龙子 (Eumeceselegans)附睾以 2 5 %戊二醛和 1%锇酸双重固定 ,按常规制作超薄切片 ,用H 6 0 0透射电镜研究观察精子的超微结构。精子由头部和尾组成 ,头部由顶体复合体和核组成 ,尾由颈段、中段、主段和末段组成。头部的顶体囊前部扁平 ,分为皮质和髓质 ,顶体下锥由类结晶状的顶体下物质组成 ,穿孔器顶端尖 ,穿孔器基板塞子状 ,细胞核延长 ,核内小管缺 ,核伸展部前端具一电子透明区 ,核肩圆 ,核陷窝锥形。颈段具片层结构 ,近端中心粒和远端中心粒的长轴呈直角 ,9束外周致密纤维与远端中心粒相应的 9束三联微管相联 ,向后与轴丝相应的 9束双联微管相联 ,中央纤维与 2个中央单微管相联。中段短 ,含有线状嵴的柱状线粒体 ,由连续的规则小卵状或小梯形致密体组成线粒体间的环状结构 ,纤维鞘伸入中段 ,终环紧贴于细胞膜的内表面。线粒体与环状结构的模式为 :rs1/mi1,rs2 /mi2 ,rs3/mi3,rs4 /mi4 ,横切面上每圈线粒体数目为 10个。主段前面部分具薄的细胞质颗粒区。纤维 3和 8至主段前端消失。轴丝复合体呈“9+2”型。蓝尾石龙子精子超微结构与已描述的石龙子科种类比较发现 ,与蜓蜥群和胎生群的石龙子相似 ;但没有发现石龙子科精子的独征。We studied the ultrastructure of epididymal spermatozoa of Eumeces elegans.After epididymal tissues of E.elegans were double fixed with 2.5% glutaric dialdehyde and 1% osmic acid,the ultrathin section were observed under H-600 transmission electron microscopy.The spermatozoa contain two major components,a head and a tail.The head contains an acrosomal complex and a nucleus,and the tail a neck,a midpiece,a principal piece and an endpiece.The mature spermatozoa are characterized by:a depressed acrosome at the anterior portion;an acrosomal vescile divided into cortex and medulla;a subacrosomal cone filled with paracrystalline subacrosomal material;a pointed prenuclear perforatorium;a stopper-like perforatorium base plate;an elongated nucleus;an epinuclear lucent zone;rounded nuclear shoulders;a coned nuclear fossa;a bilateral stratified laminar structure within neck region;absence of a endonuclear canal;a long axis of the proximal centriole almost vertical to that of the distal centriole;nine peripheral dense fibres connecting inward with the corresponding 9 triplets of the distal centriole,and backward with the doublets of the axoneme;central fibres connecting with the two central singlets;a short midpiece;columnar mitochondria with linear cristae;each intermitochondrial ring structure consisting of the complete ring of small regularly ovoid or small trapezoid dense bodies;a fibrous sheath into the midpiece;an annulus closely attached to the inner surface of the plasma membrane;the arrangement pattern of the ring structure and mitochondria in rs1/mi1,rs2/mi2,rs3/mi3,and rs4/mi4;mitochondria in order of 10 in transverse section;a thin zone of cytoplasm at the anterior portion of the principal piece;the enlarged fiber 3 and fiber 8 disappearing at the anterior portion of the principal piece;the axoneme complex having an usual 9+2 pattern.The sperm ultrastructure of E.elegans is similar to that of the species within the Sphenomorphus and the Egernia groups,but evidence of spermatozoal autapomorphies is not found in E.

关 键 词：精子 线粒体 超微结构 颈段 顶体 头部 微管 蓝尾石龙子 石龙子科 胎生 

分 类 号：Q952[生物学—动物学]