体细胞克隆猪的研究进展  

Progress of Porcine Somatic Cloning

作　　者：胡军和[1] 杨春荣[1] 雷安民[1] 敬晓棋[1] 窦忠英[1] 

机构地区：[1]西北农林科技大学国家干细胞工程研究中心陕西分中心,杨凌712100

出　　处：《中国生物工程杂志》2004年第9期21-25,共5页China Biotechnology

基　　金：国家"973"计划资助项目 (G19990 5 43 3 0 1)

摘　　要：哺乳动物克隆不仅可以保护濒危动物 ,而且与干细胞工程技术相结合能应用于克隆性治疗 ,是目前生物技术领域研究的热点之一 ,具有重大的科学意义和应用价值。由于体细胞克隆猪在人类器官移植方面具有巨大的应用潜力 ,已成为当今体细胞克隆研究的热点。从 2 0 0 0年开始 ,在不同国家相继诞生了体细胞克隆猪及转基因猪 ,但是其成功效率很低 ( 1 %～ 2 % )。主要综述了影响体细胞克隆猪的几个因素 ,涉及胞质受体、供核细胞、显微操作。The cloning of mammalian is not only used to protect extinct animals ,but also applied for therapeutic cloning,so it currently becomes one of hot points of bio-technology fields and has great scientific significance and applied value.Because the research of porcine somatic cloning has huge potential for xenotransplantation,it has been the hottest point of mammalian somatic cloning.From 2000,somatic cloning pig and transgenic pig successively born in different countries,but the efficiency is very low(1%～2%).Several factors which influencing somatic cloning of pig,such as cytoplast recipients,donor cells,micromanipulation,activation and reconstituted embryo transfer were reviewed.;

关 键 词：移植 显微操作 核细胞 巨大 克隆性 性治疗 受体 体细胞克隆 胞质 转基因猪 

分 类 号：Q785[生物学—分子生物学] S814[农业科学—畜牧学]