从原始生殖细胞分离克隆鸡胚胎生殖细胞的研究  

Isolation and Cloning of Chicken Embryonic Germ Cells from Primordial Germ Cells

作　　者：王娟[1] 杨娜娜[2] 杜立新[3] 

机构地区：[1]滨州医学院,滨州256603 [2]山东农业大学动物科技学院,泰安271018 [3]中国农业科学院畜牧所,北京100094

出　　处：《中国生物工程杂志》2004年第9期69-73,共5页China Biotechnology

基　　金：山东省自然科学基金资助项目 (Z99D0 3 )

摘　　要：从孵化 5 5天的鸡胚生殖腺中分离得到大量原始生殖细胞 (PGCs)集落 ,这些集落的细胞经多次克隆传代具有胚胎生殖细胞 (EG)的诸多特征 ,如有连续传代的能力 (传至第 9代 ) ,细胞集落有典型鸟巢状结构 ,PAS染色阳性 ,AKP染色阳性 ,在无饲养层无分化抑制因子LIF时可以自发分化成几种细胞类型 ,包括成纤维细胞、神经细胞、自律细胞等 ,悬浮培养时具有形成类胚体的能力。上述发现表明该细胞具EG细胞的诸多特性 。Numerous primordial germ cell colonies were derived from gonadals of 5.5-day-old chicken embryos.The colonies had some characteristics of embryonic germ cells,such as the ability to be continuously passed (passage 9) and the morphology of nest-like.They continued to be periodic acid and Shiff’(PAS) positive.At the same time they had the character of alkaline phosphatase (AKP) positive.The colonies would differentiate into several kinds of cells under the condition of devoid of LIF and feeder layer cells,such as fibroblast cells,neurocytes,autorhythmic cells,etc.When cultured in suspension,they successfully formed embryoid bodies.All of which indicate that the cells we obtained were pluripotent and EG-like cells.;

关 键 词：集落 胚胎生殖细胞 阳性 原始生殖细胞 成纤维细胞 PAS染色 鸡胚 分离克隆 孵化 传代 

分 类 号：Q959[生物学—动物学] R329[医药卫生—人体解剖和组织胚胎学]