板蓝根抗内毒素活性部位研究(Ⅱ)  

Effective part of anti-endotoxic activity from Radix Isatidis (Ⅱ)

作　　者：刘云海[1] 方建国[1] 王文清[1] 汤杰[1] 施春阳[1] 伍三兰[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,武汉430030

出　　处：《中南药学》2004年第5期263-266,共4页Central South Pharmacy

基　　金：国家自然科学基金课题(No39170877;39800193;39870872);卫生部科学研究基金课题(No98-2-110)

摘　　要：目的 探讨板蓝根F022部位抗内毒素活性。方法 用板蓝根F022部位溶液分别作对LPS致小鼠死亡的影响、对LPS诱导P38MAPK活性的影响及其化学成分丁香酸对内毒素的破坏作用,检测其抗内毒素活性。结果 板蓝根F022部位可降低LPS致小鼠死亡率;可抑制LPS诱导鼠单核细胞分泌P38丝裂原活化蛋白激酶活性且有剂量依赖性;板蓝根F022部位的丁香酸成分可破坏内毒素,破坏率达83.16%。结论 体内外有多项实验显示板蓝根F022部位有抗内毒素活性,该部位为板蓝根抗内毒素活性物质。OBJECTIVE To discuss the anti-endotoxic activity of F022 from Radix Isalidis. METHODS Use F022 from Radix Isatidis with samples from three groups respectively to determine (Ⅰ) effect of LPS leading to the death of mice. (Ⅱ) effect on P38MAPK activity, and (Ⅲ) effect of syringic acid ruined endotoxin. RESULTS F022 from Radix Isatidis could lower the mortality of mice and inhibit the activation of P38MAPK stimulated by LPS in dosage dependent manner. Syringic acid in F022 from radix Isatidis could ruin endotoxin. The rate of ruin was up to 83. 16%. CONCLUSIONS Some projects showed that F022 from Radix Isatidis had anti-endotoxic activity, which is the anti-en-dotoxic part of Radix Isatidis.

关 键 词：抗内毒素活性 部位 板蓝根 LPS诱导 丁香酸 小鼠 P38丝裂原活化蛋白激酶 影响 目的 显示板 

分 类 号：R285.5[医药卫生—中药学] R284.1[医药卫生—中医学]