γ线诱发的白血病小鼠胸腺细胞SHP-2 mRNA变化的研究  

Changes of SHP-2 mRNA of thymus cells of mice with γ ray-induced leukemia

作　　者：黄定德[1] 陈杞[2] 韩玲[2] 蔡建明[2] 

机构地区：[1]第三军医大学西南医院核医学科,重庆400038 [2]第二军医大学海军医学系放射医学教研室,上海200433

出　　处：《第三军医大学学报》2004年第20期1809-1811,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 ( 30 170 2 82 )~~

摘　　要：目的　探讨蛋白酪氨酸磷酸酶SHP 2mRNA在γ射线体外诱发的白血病小鼠胸腺细胞中的变化情况 ,为进一步从细胞信号转导途径入手研究辐射致癌的分子机制奠定一定的基础。方法　实验分为癌变组、未癌变组及对照组 ,应用PCR SSCP、DNA测序、荧光定量PCR法分别检测各组胸腺细胞SHP 2mRNA的突变及含量变化情况。结果　①PCR SSCP示 9/14例癌变组胸腺细胞SHP 2mRNA的第 70 0～ 10 96bp间可能发生突变 ,但DNA测序结果不支持 ;②癌变组胸腺细胞SHP 2mRNA的含量与对照组及未癌变组比无明显变化。结论　在γ射线诱发的白血病小鼠胸腺细胞中存在SHP 2的翻译异常 ,表现为翻译增强现象。Objective To investigate the alterations of SHP-2 mRNA of thymus cells of mice with γ ray-induced leukemia. Methods BALB/c mice were randomly divided into canceration group, non-canceration group, and control group. The gene mutation and content of SHP-2 mRNA in thymus cells were detected by PCR-SSCP, DNA sequencing, and real time fluorescence quantitative PCR (FQ-PCR). Results PCR-SSCP showed there was gene mutation within 700～1 096 bp of SHP-2 mRNA in thymus cells of mice in the canceration group, which was not supported by DNA sequencing. No significant difference in change of SHP-2 mRNA content was found in thymus cells in canceration, non-canceration, and control groups. Conclusion There is translational abnormality of SHP-2 mRNA in thymus cells of mice with γ ray-induced leukemia, which is presented as translational enhancement.

关 键 词：辐射致癌 信号转导 SHP-2 小鼠 白血病 

分 类 号：R322.53[医药卫生—人体解剖和组织胚胎学] R363.121[医药卫生—基础医学]