人参银杏复方制剂对缺氧复氧后大鼠海马CA1区NOS表达的影响  

THE EFFECT OF GINSENG-GINKGO COMPOUND ON NOS EXPRESSION IN CA1 AREA OF HIPPOCAMPUS FOLLOWING HYPOXIA/REOXYGENATION

作　　者：倪衡建[1] 张志军[1] 凌树才[2] 徐慧君[1] 董玉林[1] 石中瑗[3] 

机构地区：[1]南通医学院神经生物学研究室,南通226001 [2]浙江大学医学院 解剖学教研室 [3]上海交通大学海洋水下工程科学研究院

出　　处：《神经解剖学杂志》2004年第5期453-458,共6页Chinese Journal of Neuroanatomy

基　　金：交通部西部开发重点项目(2002年交通部指令性计划)

摘　　要：本文旨在观察预防性应用人参银杏复方制剂对缺氧24 h,复氧0、24、72 h时段海马CA1区锥体细胞层神经元Nissl染色变化及海马CA1区一氧化氮合酶(NOS)阳性细胞变化的影响。应用低压氧舱仿海拔8000米高空缺氧模型,以Nissl染色及还原型尼克酰胺腺嘌呤二核苷酸脱氢酶(NADPH-d)组织化学方法并结合图像分析等技术进行研究。结果表明:人参银杏复方制剂能防止缺氧24 h、复氧72 h海马CA1区锥体细胞层神经元的丢失,并能减少缺氧24 h、复氧0、24、72 h时段NOS阳性细胞数量。本研究结果提示:人参银杏复方制剂对缺氧复氧后海马CA1区神经元具有保护作用,其保护作用可能与抑制缺氧复氧后海马CA1区NOS表达有关。The effects of ginseng-ginkgo compound were studied on the morphology of pyramid neurons and the number of NOS-positive neurons in CAl area of hippocampus of the rats after hypoxia for 24 h followed by reoxygenation for 0, 24 and 72 h, by using Nissl staining, NADPH-diaphorase histochemistry and image analysis technique. The hypoxia models that mimic 8000 miter high lever were established in hypobaric oxygen chamber. The results showed that ginseng-ginkgo compound could decrease the loss of pyramid neurons in CAl area of hippocampus after hypoxia for 24 h followed by reoxygenation for 72 h and reduce the number of NOS-positve neurons in CAl area of hippocampus after hypoxia for 24 h followed by reoxygenation for 0, 24 and 72 h. The results suggested that ginseng-ginkgo compound might play a neuroprotective role in the course of hypoxia/re oygenation-induced brain injury. The neuroprotective function may be related to the inhibition of NOS expression in CAl area of hippocampus.

关 键 词：复方制剂 缺氧复氧 一氧化氮合酶(NOS) 人参 海马CA1区 神经元 海马CAL区 银杏 还原型 锥体细胞 

分 类 号：R285[医药卫生—中药学]