Kv3.1和Kv3.2钾离子通道蛋白在大鼠神经系统的分布  

LOCALIZATION OF Kv3. 1 AND Kv3. 2 POTASSIUM CHANNEL PROTEINS IN THE RAT NERVOUS SYSTEM

作　　者：周亮[1] 黄静[1] 李云庆[1] 武胜昔[1] 

机构地区：[1]第四军医大学基础医学部解剖学教研室梁铢琚脑研究中心,西安710032

出　　处：《神经解剖学杂志》2004年第5期468-474,共7页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(No.30270438)资助项目

摘　　要：本研究用免疫组织化学方法观察了Kv3.1和Kv3.2钾离子通道蛋白在大鼠神经系统的分布状况。结果显示,两种蛋白在神经系统内具有区域性的分布特点。Kv3.1免疫阳性神经元主要见于大脑皮层、海马锥体细胞层、纹状体、丘脑网状核、下丘脑、腹侧耳蜗核、桥核等脑区;Kv3.2阳性神经元主要分布于大脑皮层深层、海马始层、Calleja岛、外侧丘系腹侧核、背侧耳蜗核、小脑皮层Purkinje细胞层、前庭核等处。Kv3.1和Kv3.2阳性胞体在某些脑区如大脑皮层、海马、耳蜗核、外侧丘系核等处呈现"互补"性的分布特点。另外,在一些脑区还观察到了Kv3.1和Kv3.2免疫阳性纤维和终末。:Kv3.1和Kv3.2在神经系统内广泛的分布,提示它们可能参与了多种生理功能。Immunocytochemical staining was used to observe the distribution of Kv3. 1 and Kv3. 2 potassium channel proteins in the rat nervous system. The results showed a region-specific distribution pattern of these two proteins in the nervous system. Kv3. 1-immunoreactive neurons were mainly observed in the cortex, pyramidal cells layer of hippocampus, striatum, reticular nucleus of thalamus, hypothalamus, ventral cochlear nucleus, pontine nuclei; While Kv3. 2- immunoreactive neurons were mainly distributed in the deep layers of cortex, oriens layer of hippocampus, island of Calleja, ventral nucleus of lateral lemniscus, dorsal cochlear nucleus, Purkinje cell layer of cerebellum, vestibular nucleus. Kv3. 1 and Kv3. 2-positive neurons showed a 'reciprocal' distribution characteristics in some regions, such as cortex, hippocampus, cochlear nucleus and nucleus of lateral lemniscus. Kv3. 1 and Kv3. 2-immunoreactive fibers and terminals were also observed in some regions. The present results demonstrate that Kv3. 1 and Kv3. 2 potassium channel proteins are distributed widely in the nervous system, suggesting their involvement in multiple physiological functions.

关 键 词：神经系统 耳蜗核 大脑皮层 脑区 钾离子通道 大鼠 蛋白 细胞 前庭核 阳性神经元 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]