植物遗传转化中抑菌剂的选择  

Bacteriostatic agent selection in plant genetic transformation

作　　者：徐淑红[1] 徐香玲[1] 

机构地区：[1]哈尔滨师范大学生物系,哈尔滨150080

出　　处：《植物研究》2004年第4期491-494,共4页Bulletin of Botanical Research

基　　金：黑龙江省教育厅一般项目 (项目编号 :10 5 3 10 71)资助;黑龙江省自然基金攻关课题 (项目编号 :2 3 0 10 3 3 90 5 2 2 3 9)资助

摘　　要：农杆菌介导法进行植物遗传转化 ,在农杆菌和外植体共培养后 ,为使转化的外植体正常生长 ,必须选择适宜的抑菌剂进行除菌。本文通过抑菌圈试验和稀释法试验测试了发根农杆菌pRi1 5 834、pRiA4b、pRi1 72 4、pRiA1 3对三种头孢霉素的敏感性 ,其中头孢曲松钠的抑菌效果最好 ,最适宜浓度为 1 0 0～ 30 0mg/L。As plant genetic transformation is being carried on by Agrobacterium-mediated, a proper bacteriostatic agent must be selected to eliminate bacteria in order to make transformed explant grow in normal way after co-culturing Agrobacterium and explant. We have studied sensitivities of Agrobacterium rhizogene pRi15834、pRiA 4b、pRi1724、pRiA13 to three kinds of cephalosporin by bioactivity and dilution tests. Ceftriaxone sodium has the best bactericidal effect of them, the proper concentration is 100～ 300 mg/L

关 键 词：遗传转化 外植体 农杆菌介导法 适宜浓度 抑菌圈 植物 抑菌剂 正常 头孢曲松钠 共培养 

分 类 号：Q943[生物学—植物学]