紫草宁生物合成相关的代谢酶及基因  

The Metabolic Enzymes and Genes Related to Shikonin Biosynthesis in Cultured Lithospermum Erythrorhizon Cells

作　　者：刘志[1] 杨永华[1] 

机构地区：[1]南京大学医药生物技术国家重点实验室,生命科学学院植物细胞与分子生物学实验室,江苏南京210093

出　　处：《药物生物技术》2004年第5期332-335,350,共5页Pharmaceutical Biotechnology

摘　　要：紫草是一种重要的中草药材,其培养细胞在M9培养基中可以大量生产主要的药物成分紫草宁。本文主要论述了在紫草细胞中与紫草宁形成相关的代谢酶和基因方面取得的研究进展,分别讨论了这些代谢酶和基因克隆的功能及表达特性,对植物次生代谢、紫草宁次生药物的生产调控及其代谢工程等研究具有重要的意义。The shikonin and its derivatives is produced industrially by suspension cultured Lithospermum erythrorhizon cells in M9 medium. The metabolic enzymes and genes related to shikonin formation as well as their functions and expressional characteristics are reviewed in this paper, which may play a significant role in studying the regulation of shikonin production, shikonin metabolic engineering and plant secondary metabolism.

关 键 词：紫草培养细胞 紫草宁 生物合成 代谢酶 基因克隆 

分 类 号：O55[理学—热学与物质分子运动论]