细胞因子IL-2、IL-10、TNF-α在小鼠单纯疱疹病毒性脑炎中的表达  

Expression of the cytokines IL-2、IL-10 and TNF-α on herpes simplex encephalitis in mice

作　　者：张敏[1] 魏桂荣[1] 梅元武[1] 董继华[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院神经内科,武汉430022 [2]华中科技大学同济医学院附属协和医院病毒室,武汉430022

出　　处：《卒中与神经疾病》2004年第5期275-278,共4页Stroke and Nervous Diseases

摘　　要：目的　检测细胞因子IL 2、IL 10、TNF α在单纯疱疹病毒性脑炎 (HSE)中的表达和变化 ,探讨细胞因子IL 2、IL 10、TNF α在HSVE发病机制中的作用。方法　使用逆转录—聚合酶链反应 (RT PCR)检测颅内感染单纯疱疹病毒 1型 (HSV1)的小鼠在感染后及使用无环鸟苷 (ACV)治疗后细胞因子IL 2、IL 10、TNF α变化及病理变化。结果　HSV1感染后出现脑内出血坏死性的病理改变 ,IL 2、IL 10、TNF α均明显上升 ;无环鸟苷治疗好转后脑内病理变化改善 ,IL 2保持稳定 ,IL 10继续上升 ,TNF α显著下降。结论　在小鼠HSVE急性期TH1型及TH2型反应同时被激活 ,发挥抗病毒作用 ,并以TH1型反应为主 ;在HSVE恢复期以TH2型反应为主 ,并抑制体内免疫反应的扩大 ;3种细胞因子的动态变化反映出机体免疫调节的动态平衡 ,并可反映HSVE的预后 ,早期应用ACV治疗HSVE确有极其明显的抗病毒治疗作用。Objective To investigate the expression of the cytokines IL-2、IL-10 and TNF-α and their roles in herpes simplex encephalitis(HSE).Methods We used semiquantitative reverse transcription polymerase chain reaction(RT PCR) to detect the mRNA of IL 2?IL 10 and TNF α in mice's brains of uninfected control and HSE and treated with acyclovir(ACV) after HSE.Pathologic slices of brain tissues of mice were used to identify their difference morphologic.Results After herpes simplex virus typel(HSV1) infection,the lesions of haemorrhage and necrosis in mice's brains could be observed by microscope,and the levels of IL 2?IL 10 and TNF α increased remarkably.While the mice were treated with ACV after HSV1 infection, the lesions in mice's brains were improved.However the level of IL 2 was still high and IL 10 increased consistently.TNF α decreased rapidly compared with that of HSV1 infected mice.Conclusions In HSE of mice,many cytokines are upregulated including IL 2?IL 10 and TNF α to eliminate virus and TH1 type response is dominant.In con 1 valescence,there is a shift in the cytokine expression profile from TH1 profile to TH2 profile and the shift can inhibit the overexpression of immune response in animals.The prognosis could be made from the changes of the three cytokines which reflect the shift balance of immunity regulation.ACV has remarkable effects on treatment of HSE of early stage.

关 键 词：IL-10 IL-2 TNF-Α 细胞因子 小鼠 单纯疱疹病毒性脑炎 表达 逆转录-聚合酶链反应 RT-PCR 保持 

分 类 号：R512[医药卫生—内科学] R714[医药卫生—临床医学]