用苯噻草胺吸附伏安法测定植物DNA  

Adsorptive voltammetric determination of DNA in plant using BTMPA

作　　者：张正奇[1] 张振乾[1] 曾伟[1] 严八妮[1] 

机构地区：[1]湖南大学化学化工学院,湖南长沙410082

出　　处：《化学传感器》2004年第3期9-12,共4页Chemical Sensors

摘　　要：提出了用苯噻草胺作为电化学指示剂，在悬汞电极上以吸附伏安法测定DNA的新方法。在pH4．4的醋酸盐缓冲溶液中，BTMPA与植物DNA形成的复合物在悬汞电极上于-1．53V左右产生一吸附伏安峰，利用此峰测定稗草DNA的线性范围为0．75μg／L～7．5×10^3μg／L，检测限低至0．75μg／L；水稻DNA线性范围为7．5mg／L～37．5mg／L，检测限为0．30mg／L；玉米DNA线性范围为5．0mg／L～50．0mg／L。检测限为0．37mg／L。In this paper, a new procedure for the adsorptive vohammetric determination of DNA in plant using BTMPA has been proposed. In a pH4.4 buffer solution, The complex of plant DNA with BTMPA yields a sensitive adsorptive voltammetric peak at-1.53 V on the HDME, which can be used for the determination of trace DNA of plant. For echinochloa the linear range is 0. 75μg/L～7.5×10~3μg/L and the detection limit is 0.75μg/L, and oriza, 7.5mg/L～37.5mg/L,0. 30mg/L and zea, 5.0mg/L～50.0mg/L,0.37mg/L.

关 键 词：定植 苯噻草胺 DNA 稗草 植物 玉米 水稻 检测限 左右 测定 

分 类 号：Q943[生物学—植物学]