纳米锰锌铁氧体的生物相容性研究  

Biocompatibility Study of Nanosized Mn-Zn Ferrite

作　　者：路新丽[1] 张东生[1] 顾宁[1] 郑杰[1] 唐秋莎[1] 金立强[1] 万美玲[1] 

机构地区：[1]东南大学基础医学院病理学与病理生理学系,生物科学与医学工程系,江苏南京210009

出　　处：《南京医科大学学报（自然科学版）》2004年第6期582-585,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助项目(30371830);江苏省自然科学基金(BK2001003);江苏省中医药;中西医结合重点项目(H027)

摘　　要：目的:研究用于肿瘤磁流体热疗的自制纳米级Mn-Zn铁氧体(Mn0.5Zn05Fe2O4)的生物相容性.方法:溴化-3(4,5-二甲基噻唑基-2)-2,5-二苯基四唑(MTT)实验评价其体外细胞毒性;溶血试验评价其有无溶血作用;小鼠腹腔注射其无菌生理盐水混悬液以测定其LD50及最大耐受量(MTD);微核试验评价其有无致畸、致突变作用等.结果:MTT显示该材料对L-929细胞毒性为0～1级;无溶血作用;昆明小鼠腹腔注射该材料混悬液1ml,在极限浓度时的LD50测不出,MTD值达到6 g/kg;微核实验结果表明无致畸致突变作用.结论:该材料是一种具有良好生物相容性的材料,应用于肿瘤热疗具有广阔前景.Objective: To evaluate the in viro and in vivo toxicity of self-prepared nanosized Mn-Zn ferrite, which has the potential implication in tumor hyperthermia. Methods: MTT assay was used to investigate the in vitro citoxicity and hemolysis test was carried out to estimate whether it had blood toxicity. Mn-Zn ferrite suspended in sterile 0.9%NaCl was intraperitoneally injected into KM mouse to calculate the LD50 and the most tolerance dose(MTD), micronucleus(MN) were reckoned to identify whether it was genotoxic. Results: The experiments showed that the toxicity of the material on mouse fibroblast(L-929) cell lines was between 0-1 degree and it had no hemolysis activity. LD50 could not be estimated after intraperitoneal injection of 1 ml suspension at the highest concentration and the MTD was 6 g/kg; Micronucleus test showed no genotoxic effects. Conclusion: The self-prepared nanosized Mn-Zn ferrite is a kind of high biocompatibility materials and is suitable for further application in tumor hyperthermia.

关 键 词：生物相容性 致突变作用 混悬液 致畸 溶血作用 MTT 腹腔注射 溴化 纳米级 苯基 

分 类 号：R285.5[医药卫生—中药学] R318[医药卫生—中医学]