昆明小鼠单细胞胚胎体外培养的研究  

STUDY ON CULTURING KUMING MOUSE 1-CELL EMBRYOS IN VITRO

作　　者：张守全[1] 孙拓[2] 

机构地区：[1]华南农业大学畜牧系 [2]北京农业大学畜牧系

出　　处：《华南农业大学学报》1993年第1期10-15,共6页Journal of South China Agricultural University

基　　金：国家自然科学基金

摘　　要：本试验研究了葡萄糖、EDTA和小鼠血清对昆明小鼠单细胞胚体外发育的影响.结果发现:不含葡萄糖HECM-1组桑椹率为40.05％,对照组为8.14％;含EDTA的CZB组桑椹率为61.18％,对照组为47.44％;小鼠血清对昆明小鼠单细胞胚体外发育无促进作用。可见,葡萄糖抑制昆明小鼠单细胞胚胎体外发育;在不含葡萄糖的条件下,EDTA支持小鼠早期胚胎体外发育;无论有无葡萄糖小鼠血清对小鼠早期胚体外发育无促进作用。This study was dealt with the effects of glucose and mouse serum and EDTA on Kuming mouse 1-cell embryo development in vitro. The results show that the precentage of morula embryos cultured in glucose free HECM-1 to 40. 05%, and the control only 8.14%, cultured in CZB containing 0.11mM EDTA is 61. 18%, the control 47. 44%. In conclusion, the presence of glucose in culture medium is detrimental to Kuming mouse 1-cell embryo development. EDTA supports mouse embryo developement in vitro in glucose -free medium also. Glucose lacked or not, mouse serum doesn't affected in vitro mouse embrvo development.

关 键 词：小鼠 胚胎 体外培养 细胞 

分 类 号：S865.133[农业科学—野生动物驯养]