紫外分光光度法测定头孢哌酮注射剂含量  

Determination of Cefoperazone Injection by UV spectrophotometry

作　　者：王雷[1] 李军 王玮[1] 

机构地区：[1]新汶矿业集团公司中心医院,新汶271233 [2]山东省药品检验所,济南250012

出　　处：《齐鲁药事》2004年第8期24-25,共2页qilu pharmaceutical affairs

摘　　要：目的　建立紫外分光光度法测定头孢哌酮注射剂含量。方法　在 2 0 0～ 4 0 0nm波长范围内扫描,确定测定波长。然后进行吸收度A与浓度C的线性关系实验;精密度测定;回收实验;稳定性实验。结果　确定 2 2 8 4nm为测定波长。头孢哌酮在浓度2 5～ 4 0 0 μg·ml-1范围内与吸收度呈良好的线性关系(r =0 9999);平均回收率为 99 6 %。结论　本法简便、准确。Objective Establish a method of determining Cefoperazone Injection by UV spectrophotometry.Methods Scanning from 200～400nm to determine the detection wavelengh.Then experiments were as follows:linear relationship between the absorbance (A) and the concentration (C),precision,recovery and stability.Results At the detection wavelength 228.4nm,the linearity was verified from 2.5～40.0μg·ml -1(r=0.9999).The mean recovery was 99.6%.Conlusion The method was convenient and accurate.

关 键 词：头孢哌酮 注射剂 紫外分光光度法 含量 吸收度 稳定性实验 精密度测定 平均回收率 线性关系 

分 类 号：R927.2[医药卫生—药学] R978