血管钠肽对人桡动脉的舒张作用及其机制  

Relaxation of human radial artery by vasonatrin peptide and its mechanism

作　　者：于军[1] 朱妙章[2] 韦耿泽[2] 陈宝莹[3] 吕顺艳[2] 郭海涛[2] 康云帆[4] 周京军[2] 

机构地区：[1]第四军医大学教学实验中心,陕西西安710032 [2]第四军医大学生理学教研室,陕西西安710032 [3]第四军医大学唐都医院放射科,陕西西安710032 [4]第四军医大学西京医院心血管外科中心,陕西西安710032

出　　处：《心脏杂志》2004年第5期407-409,413,共4页Chinese Heart Journal

基　　金：国家自然科学基金资助项目 (No.3 9970 3 2 7)。

摘　　要：目的 :研究血管钠肽 (VNP)对人桡动脉 (humanradialartery,HRA)的舒张作用及其机制。方法 :采用离体血管灌流的方法 ,观察VNP对内皮完整和去内皮HRA的舒张作用 ,以及 8 Br cGMP、NPR A、B选择性抑制剂HS 14 2 1、鸟苷酸环化酶选择性抑制剂美蓝 (methyleneblue ,MB)和Ca2 + 激活K+ 通道 (Ca2 + activatedK+ channel,KCa)的选择性抑制剂TEA对这一过程的影响。结果 :VNP(10 10 ～ 10 -6mol/L)可剂量依赖性地舒张HRA ,该作用无内皮依赖性。 8 Br cGMP(10 -7～ 10 -3 mol/L)可模拟VNP的血管舒张效应。HS 14 2 1(2× 10 -5mol/L)或MB(10 -5mol/L)完全阻断VNP舒张作用。TEA(10 -3 mol/L)减弱VNP的舒血管作用。结论 :VNP对HRA具有不依赖内皮的舒张作用 ,它主要是通过作用于NPR A、B ,即钠尿肽的鸟苷酸环化酶耦联受体 ,升高细胞内的cGMP水平。KCa可能是其舒张作用的重要效应分子。为应用VNP防治血管移植术后的痉挛提供了重要的理论依据。目的 :研究血管钠肽 (VNP)对人桡动脉 (humanradialartery,HRA)的舒张作用及其机制。方法 :采用离体血管灌流的方法 ,观察VNP对内皮完整和去内皮HRA的舒张作用 ,以及 8 Br cGMP、NPR A、B选择性抑制剂HS 14 2 1、鸟苷酸环化酶选择性抑制剂美蓝 (methyleneblue ,MB)和Ca2AIM: To investigate the vasorelaxing effect of vasonatrin pe pt ide(VNP) on human radial artery(HRA) and to reveal its underlying mechanism . METHODS: The vasorelaxing effect of VNP on HRA with and without endothelium was observed by perfusion in vitro. Effects of 8-Br-cGMP, HS- 142-1, the selective inhibitor of NPR-A and B, methylene blue (MB), the select ive inhibitor of GC, and tetraethylammonium (TEA), the selective inhibitor of Ca 2+-activated K + channel (K Ca), were also investigated. RESULTS: It was found that VNP from 10 -10 to 10 -6 mol/L indu ced a concentration- dependent relaxation of HRA, which was independent of the endothelium. This effect was mimicked by 8-Br-cGMP (10 -7～10 -3 mol / L), and was abolished by either HS-142-1 at 2×10 -5 mol/L, or MB at 10 -5 mol/L. It was also attenuated by TEA at 10 -3 mol/L. CONCLUS ION: The present study demonstrated that vasorelaxing effect of VNP on HR A is independent of endothelium, It results from an increase in the intracellul ar cGMP level by the activation of natriuretic peptide guanyl cyclase receptors in VSMCs, and K Ca is an important mediator. The study has provided new and useful information for the use of VNP in the prevention and treatment of the sp asm of transplanted blood vessels.

关 键 词：血管钠肽 人桡动脉 CGMP TEA 美蓝 

分 类 号：Q463[生物学—生理学] R33[医药卫生—人体生理学]